SW 1088[SW-1088; SW1088]（人脑星型胶质∏瘤细胞）

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品　　牌：ATCC

产品型号：HTB-12 (株)

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所在地区：上海

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SW 1088[SW-1088; SW1088]（人脑星型胶质瘤细胞）

SW 1088[SW-1088; SW1088]（人脑星型胶质瘤细胞）

Cell Biology
ATCC^® Number:	HTB-12™	Price:	$330.00
Designations:	SW 1088 [SW-1088; SW1088]	Depositors:	A Leibovitz
Biosafety Level:	1	Shipped:	frozen
Medium & Serum:	See Propagation	Growth Properties:	adherent
Organism:	Homo sapiens (human)	Morphology:	fibroblast

Source:	Organ: brain Disease: astrocytoma
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Tumorigenic:	Yes, in nude mice (The cells produce a grade III tumor.)
Antigen Expression:	Blood type A; Rh+
DNA Profile (STR):	Amelogenin: X,Y CSF1PO: 10,12 D13S317: 12 D16S539: 12,13 D5S818: 11 D7S820: 9 THO1: 7,9.3 TPOX: 8 vWA: 16,17
Cytogenetic Analysis:	hypertriploid; modal number = 72 to 74. The rate of higher ploidies was 4.2%. Most chromosomes were morphologically normal. Three marker chromosomes were common to all cells: del(1) (q11), der (9)t(7;?;9) (q11?;?;p24), and der (10)t(4;10) (q21;q15). The der (9) was paired in nearly 50% of the cells. Usually one, but occasionally three double minutes (DM) were seen in a few cells. Five copies of normal N5, N7 and N20 were seen in most cells. The X and Y were paired. The presence of Y chromosomes was confirmed in the QM stained preparation.
Isoenzymes:	AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1; Me-2, 1-2; PGM1, 1-2; PGM3, 1
Age:	72 years
Gender:	male
Ethnicity:	Caucasian
Comments:	The SW 1088 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1975 from an astrocytoma taken from a 72 year old male Caucasian. The cell line was received at the ATCC in January, 1982 at passage 23.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 100%(L-15 medium does not require CO2 to maintain pH, and should be used without CO2 gassing.)
Subculturing:	Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium renewal: 2 to 3 times per week
Preservation:	Culture medium, 95%; DMSO, 5%
References:	22539: Fogh J , et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 22551: Wright WC , et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed:

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SW 1088[SW-1088; SW1088]（人脑星型胶质瘤细胞）