| Designations: | SK-MES-1 | Depositors: | G Trempe
LJ Old | | Biosafety Level: | 1 | Shipped: | frozen | | Medium & Serum: | See Propagation | Growth Properties: | adherent | | Organism: | Homo sapiens (human) | Morphology: | epithelial | | | | Source: | Organ: lung
Disease: squamous cell carcinoma
Derived from metastatic site: pleural effusion | | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | | Related Cell Culture Products | | Restrictions: | The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439. | | Antigen Expression: | Blood Type O; Rh+; HLA A3, Aw30, B7, B27 | DNA Profile
(STR): | Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 13
D5S818: 11
D7S820: 8
THO1: 6,9.3
TPOX: 8
vWA: 14 | Cytogenetic
Analysis: | The stemline chromosome number is hypotriploid, with the 2S component occurring at 3.2%. Seventeen to 20 marker chromosomes were common to most S metaphases. Normal X, 13, and 19 chromosomes were absent, and chromosomes 2, 3, 14, 17 and 20 were generally monosomic. The Y chromosome was not detected using QM staining. | | Isoenzymes: | AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1; Me-2, 1-2; PGM1, 1-2; PGM3, 1 | | Age: | 65 years adult | | Gender: | male | | Ethnicity: | Caucasian | | Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% | | Subculturing: | Protocol: Remove medium, rinse with fresh 0.25% trypsin, 0.03% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium renewal: 2 to 3 times per week | | Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase | | References: | 21869: Fogh J, editor. Human tumor cells in vitro. New York: Plenum Press; 1975, pp. 115-159.
22536: Fogh J , et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
23226: Pollack MS , et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: | |
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