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Related Cell Culture Products
Strain:
C3H
Age:
4 weeks
Comments:
Sol8 is a myogenic cell line isolated by Daubas et al. from primary cultures of soleus muscle taken from the leg of a normal C3H mouse. The cells were split two times and then cloned by limiting dilution. Sol8 cells will differentiate into myotubes with serum deprivation. They have a phenotype similar to slow twitch fibers. Note: do not allow the cells to become confluent. The myoblast component of the population will rapidly become depleted if the culture becomes confluent.
Propagation:
ATCC complete growth medium: Dulbeccos modified Eagles medium with 4.5 g/L glucose, 80%; fetal bovine serum, 20%
Subculturing:
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Subculture at 50% of confluence. Subcultivation ratio: A subcultivation ratio of 1:8 to 1:12 is recommended
Medium renewal: 2 to 3 times per week
Preservation:
Culture medium, 95%; DMSO, 5%
Doubling Time:
16 to 18 hrs
References:
23248: Daubas P , et al. Functional activity of the two promoters of the myosin alkali light chain gene in primary muscle cell cultures: comparison with other muscle gene promoters and other culture systems. Nucleic Acids Res. 16: 1251-1271, 1988. PubMed:
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