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                NOR-10(小鼠骨骼肌成纤维细胞)

                NOR-10(小鼠骨骼肌成纤维细胞)
                <
                • NOR-10(小鼠骨骼肌成纤维细胞)
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                ATCC
                CCL-197 (株)
                上海锐聪科技发↑展有限公司
                上海
                详细说明

                NOR-10(小鼠骨骼肌成纤维细胞)

                NOR-10(小鼠骨骼肌成纤维细胞)
                 
                Cell Biology
                ATCC® Number: CCL-197™ Price: $330.00
                Designations:
                NOR-10
                Depositors:
                BC Starcher
                Biosafety Level:
                1
                Shipped:
                frozen
                Medium & Serum:
                See Propagation
                Growth Properties:
                adherent
                Organism:
                Mus musculus (mouse)
                Morphology:
                fibroblast
                 
                Source:
                Tissue: skeletal muscle
                Cell type: fibroblast
                Disease: normal
                Permits/Forms:
                In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
                Reverse Transcript:
                negative
                Antigen Expression:
                H-2b
                Strain:
                C57BL/10
                Age:
                30 days
                Comments:
                This may be used as a matched control for ATCC CCL-198 (BLO-1).
                Tested and found negative for ectromelia virus (mousepox).
                Propagation:
                ATCC complete growth medium: Dulbeccos modified Eagles medium, 80%; fetal bovine serum, 20%
                Subculturing:
                Remove medium, add fresh 0.1% trypsin for about 10 minutes, dislodge cells and centrifuge to remove from trypsin. Add fresh medium to cell pellet, resuspend cells in fresh medium and dispense into new flasks. Subculture every 3 to 5 days.
                Subcultivation ratio: A subcultivation ratio of 1:2 to 1:5 is recommended

                Medium renewal: 2 to 3 times per week

                 

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