Tissue: skeletal muscle Cell type: fibroblast Disease: normal
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Reverse Transcript:
negative
Antigen Expression:
H-2b
Strain:
C57BL/10
Age:
30 days
Comments:
This may be used as a matched control for ATCC CCL-198 (BLO-1). Tested and found negative for ectromelia virus (mousepox).
Remove medium, add fresh 0.1% trypsin for about 10 minutes, dislodge cells and centrifuge to remove from trypsin. Add fresh medium to cell pellet, resuspend cells in fresh medium and dispense into new flasks. Subculture every 3 to 5 days. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:5 is recommended