| Designations: | NCI-H596 [H596] | Depositors: | AF Gazdar
JD Minna |
| Biosafety Level: | 1 | Shipped: | frozen |
| Medium & Serum: | See Propagation | Growth Properties: | adherent |
| Organism: | Homo sapiens (human) | Morphology: | epithelial |
| |
| Source: | Organ: lung
Disease: adenosquamous carcinoma |
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| Related Cell Culture Products |
| Isolation: | Isolation date: 1983 |
| Tumorigenic: | Yes, in nude mice |
DNA Profile
(STR): | Amelogenin: X
CSF1PO: 12,13
D13S317: 11
D16S539: 11
D5S818: 11,13
D7S820: 11
THO1: 7,9.3
TPOX: 8
vWA: 16 |
Cytogenetic
Analysis: | modal number = 71; range = 65 to 75.
This is a near triploid human cell line. The modal chromosome number was 71 although cells with 70 chromosomes occurred frequently. The frequency of higher ploidies was 2.9%.; Over 11 marker chromosomes were common to all cells, and at least 6 others were found in some cells. Among the common markers were: del(1)(p22), i(3q), i(6p), i(7p), t(7p17q), 14q+ and five others.; Of these, del(1) and i(7p) were generally double [occasionally triple for del(1)]. Constantly, there were 2 to 3 minute chromosomes that were about 1/2 the size of a G group chromosome.; Structurally normal N17 had only a single copy per cell, whereas N15 had four copies and N20 and N21 had four or more copies. The X chromosome was paired, but the Y was not found by fluorescence microscopy. |
| Isoenzymes: | AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1; Me-2, 0; PGM1, 1; PGM3, 1 |
| Age: | 73 years |
| Gender: | male |
| Ethnicity: | Caucasian |
| Comments: | The NCI-H596 cell line was derived by A.F. Gazdar and H. Oie in 1983 from a tumor mass in the chest wall of a patient with adenosquamous carcinoma of the lung.
The specimen was obtained prior to therapy.
The cells express easily detectable levels of p53 mRNA (comparable to levels found in normal lung), and exhibit no gross structural DNA abnormalities.
The cells stain positively for keratin and vimentin, but are negative for neurofilament triplet protein.
The cells express multiple markers of squamous differentiation, including cross linked envelope formation, transglutaminase activity and production of involucrin. |
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0C |
| Subculturing: | Protocol: - Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium renewal: 2 to 3 times per week
|
| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2001
recommended serum: ATCC 30-2020 |
| References: | 1605: Banks-Schlegel SP , et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed:
1806: Takahashi T , et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed:
22437: Levitt ML , et al. Cross-linked envelope-related markers for squamous differentiation in human lung cancer cell lines. Cancer Res. 50: 120-128, 1990. PubMed: |
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