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Tumorigenic:
Yes,
Cytogenetic Analysis:
modal number = 40
Strain:
Swiss
Age:
fetus fetus
Comments:
When confluent, the cells fuse to form multinucleated myotubes. They are slightly responsive to acetylcholine. The myoblastic component of the population will rapidly become depleted if the cells are allowed to become confluent, since more extensive fusion will take place. To avoid this, the cells should be subcultured before they become confluent, and they should be recloned periodically with selection for myoblastic clones.
Propagation:
ATCC complete growth medium: Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10% Temperature: 37.0C
Subculturing:
Protocol: Remove medium, add fresh 0.25% trypsin, 0.53mM EDTA solution for 2 to 5 minutes, remove trypsin, add fresh medium, aspirate and dispense into new Bovine Collagen type l (0.03 mg/ml) coated flasks. Differentiation is enhanced by reducing the sera to 2% each.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium renewal: Twice per week
Doubling Time:
17 to 19 hrs
References:
1063: Christian CN , et al. Synapse formation between two clonal cell lines. Science 196: 995-998, 1977. PubMed: 193191
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