| Designations: | SW 900 [SW-900; SW900] | Depositors: | A Leibovitz |
| Biosafety Level: | 1 | Shipped: | frozen |
| Medium & Serum: | See Propagation | Growth Properties: | adherent |
| Organism: | Homo sapiens (human) | Morphology: | epithelial |
| |
| Source: | Organ: lung
Disease: squamous cell carcinoma
Tumor stage: grade IV |
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| Related Cell Culture Products |
| Tumorigenic: | Yes, produces tumors in nude mice consistent with type II broncheolar adenocarcinoma |
| Antigen Expression: | Blood type A; Rh+ |
DNA Profile
(STR): | Amelogenin: X
CSF1PO: 11
D13S317: 8
D16S539: 11
D5S818: 11
D7S820: 11,12
THO1: 8
TPOX: 11
vWA: 16 |
Cytogenetic
Analysis: | hypotriploid; modal number = 56. The rate of higher ploidies was 21.8%. Cells in this line have complex karyotypes.
Over 30 marker chromosomes were found in 5 metaphase karyotypes. The marker chromosomes, t(1q;10q), t(1q;22q), t(2q;3q), der (3)t(2;3)(p11;q29), der (7)t(7;17) (q36;q21) and about 20 others were common to most cells.
Among these der (7) was generally paired. Normal N3, N5, N12, N13, N14 and N17 were absent. The X was single and the Y was not found on fluorescence observation. |
| Isoenzymes: | AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 2; PGM1, 1; PGM3, 2 |
| Age: | 53 years |
| Gender: | male |
| Ethnicity: | Caucasian |
| Comments: | The SW 900 cell line was initiated by A. Leibovitz in January 1975 from biopsy tissue of a squamous cell carcinoma.
The tumor originated in the lung of a 53 year old male Caucasian.
The histopathology of the surgical specimen was determined to be grade IV carcinoma.
A frozen ampule at passage 15 was received at the ATCC in January, 1982. |
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
| Subculturing: | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: A subcultivation ratio of 1:2 is recommended
Medium renewal: 2 to 3 times per week |
| Preservation: | culture medium 95%; DMSO, 5% |
| Related Products: | recommended serum: ATCC 30-2020 |
| References: | 22536: Fogh J , et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J , et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
22551: Wright WC , et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: |
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