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产品报价： 面议

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产品热度：加载中

品　　牌：ATCC

产品型号：CRL-12557 (株)

制造厂商：上海锐聪科技发展有限公司

所在地区：上海

7F2（小鼠杂交瘤细胞）

Cell Biology ATCC® Number: CRL-12557™ Price: $330.00 Designations: 7F2 Depositors: ZymoGenetics, Inc. Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: adherent Organism: Mus musculus (mouse) Morphology: fibroblast   Source: Organ: bone marrow Cell type: osteoblast Cellular Products: alkaline phosphatase Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. This material is cited in a U.S. and/or other Patent or Patent Application, and may not be used to infringe on the patent claims. Related Cell Culture Products Age: 5 to 6 weeks Gender: male Comments: 7F2 is a spontaneously immortalized cell line isolated from p53-/- mouse bone marrow taken from the femur and subsequently cloned. [47440] The cells appeared to correspond to mature osteoblasts because they express alkaline phosphatase, secrete type I collagen, show a significant cyclic adenosine monophosphate response to parathyroid hormone, secrete osteocalcin, and mineralize extensively. [53586] However, in contrast to mature osteoblasts, this clone can be induced to undergo massive adipocyte differentiation, and this differentiation is accompanied by the complete loss of expression of all osteoblastic markers except alkaline phosphatase. [53586] These observations indicate that some cells that have acquired all of the characteristics of mature osteoblasts can be diverted to the adipocyte pathway. [53586] Propagation: ATCC complete growth medium: Alpha minimum essential medium with 2 mM l-glutamine and 1 mM sodium pyruvate without ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10% Temperature: 37.0C Subculturing: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks. Subculture before the cells become confluent. Subcultivation ratio: A subcultivation ratio of 1:3 to 1:10 is recommended Medium renewal: Every 2 to 3 days Preservation: culture medium 95%; DMSO, 5% Related Products: recommended serum: ATCC 30-2020 References: 47440: Moore EE . Preparation of immortalized cells. US Patent 5,830,682 dated Nov 3 1998 53586: Thompson DL , et al. The derivation and characterization of stromal cell lines from the bone marrow of p53-/- mice: new insights into osteoblast and adipocyte differentiation. J. Bone Miner. Res. 13: 195-204, 1998. PubMed: