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                HIT-T15（仓鼠beta胰岛细胞）
                
                
                
                    
                
                        
                
                            
                HIT-T15（仓鼠beta胰岛细胞）
                
                            
                
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                                                    HIT-T15（仓鼠beta胰岛细胞）
                                                
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                ATCC
                
                            
                CRL-1777 (株)
                
                            
                上海锐聪科技发展有限公司
                
                            
                            
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                HIT-T15（仓鼠beta胰岛细胞）
                            
                                        
                HIT-T15（仓鼠beta胰岛细胞） 
                                            		        
                        
                                     
                        
                                        
                                                    
                                                                
                Cell Biology
                                                        		                    
                                    
                                        
                                            ATCC® Number:                            CRL-1777™                                 Price:                            $203.00                        
                                                        
                                                                
                Designations:
                                                        		                                                
                HIT-T15
                                                        		                                                
                Depositors:
                                                        		                                                
                R Santerre
                                                        		                    
                                    
                                                                
                Biosafety Level:
                                                        		                                                
                2 [Cells Contain PAPOVAVIRUS]
                                                        		                                                
                Shipped:
                                                        		                                                
                frozen
                                                        		                    
                                    
                                                                
                Medium & Serum:
                                                        		                                                
                See Propagation
                                                        		                                                
                Growth Properties:
                                                        		                                                
                adherent
                                                        		                    
                                    
                                                                
                Organism:
                                                        		                                                
                Mesocricetus auratus (hamster, Syrian golden)
                                                        		                                                
                Morphology:
                                                        		                                                
                epithelial
                                        HIT-T15（仓鼠beta胰岛细胞）
                                                        		                    
                                    
                                                             
                                    
                                        Source:                                                
                Organ: pancreas
                                        Tissue: islet of Langerhans
                                        Cell type: beta cell
                                                        		                    
                                    
                                        Cellular Products:                                                
                insulin
                                                        		                    
                                    
                                        Permits/Forms:                                                
                In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
                                                        		                    
                                    
                                                            
                                    
                                        Applications:                                                
                transfection host (Roche FuGENE® Transfection Reagents)
                                                        		                    
                                    
                                        Receptors:                                                
                glucagon; somatostatin; glucocorticoid
                                                        		                    
                                    
                                        Comments:                                                
                This line was established from a primary culture of Syrian hamster islet cells which were transformed with SV40.
                                        The cells secrete up to 24 ng of insulin per 10 exp6 cells per 24 hours (passage 60).
                                        Insulin secretion is stimulated by glucose and glucagon, and is suppressed by somatostatin and glucocorticoids.
                                        Insulin synthesis decreases with length of time in culture.
                                                        		                    
                                    
                                        Propagation:                                                
                ATCC complete growth medium: Hams F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 87.5%; dialyzed horse serum, 10%; fetal bovine serum, 2.5%
                                        Temperature: 37.0C
                                        Atmosphere: air, 95%; carbon dioxide (CO2), 5%
                                                        		                    
                                    
                                        Subculturing:                                                
                Protocol:                        
                                              
                1. Remove and discard culture medium.
                  2.                             
                2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
                  3.                             
                3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
                                              Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
                  4.                             
                4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
                  5.                             
                5. Add appropriate aliquots of the cell suspension to new culture vessels.
                  6.                             
                6. Incubate cultures at 37°C.
                  7.                         
                                        
                                        Interval: An inoculum of approximately 1.3 X 10(5) viable cells per sq. cm is recommended.
                                        
                                        Medium renewal: 2 to 3 times per week
                                                        		                    
                                    
                                        Preservation:                                                
                Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
                                        Storage temperature: liquid nitrogen vapor phase
                                                        		                    
                                    
                                        Related Products:                                                
                Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2004
                                        recommended serum: ATCC 30-2020
                                                        		                    
                                    
                                        References:                                                
                22694: Lord JM , Ashcroft SJ . Identification and characterization of Ca2+-phospholipid-dependent protein kinase in rat islets and hamster beta-cells. Biochem. J. 219: 547-551, 1984. PubMed: 
                                        23275: Santerre RF , et al. Insulin synthesis in a clonal cell line of simian virus 40-transformed hamster pancreatic beta cells. Proc. Natl. Acad. Sci. USA 78: 4339-4343, 1981. PubMed: 
                                        23561: Aguilar-Bryan L , et al. Co-expression of sulfonylurea receptors and KATP channels in hamster insulinoma tumor (HIT) cells. Evidence for direct association of the receptor with the channel. J. Biol. Chem. 267: 14934-14940, 1992. PubMed: 
                                                        		                    
                                                            
                                            		        
                    
                
                
                
                
                
                
                    
                
                        
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