Background: Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca2+ sensitivity of smooth muscle contraction.
Immunogen: Recombinant full length protein of active RhoA
Tested applications: IP, IHC
Recommended dilutions:1 μg for 1~2 mg total cellular proteins
Concentration: 1 mg/ml
Host: mouse
Clonality: Monoclonal
Isotype: IgG
Purity: Purified from ascites
Format: Liquid
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti-active RhoA antibody recognizes active RhoA from vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
IP/WB analysis of active RhoA protein. Purified recombinant RhoA proteins were loaded with
GDP (lane 1) or GTPgS (lane 2). These proteins were immunoprecipitated with anti-active RhoA mouse monoclonal antibody (Cat. # 26904).After SDS/PAGE, the membrane filter was
probed with anti-RhoA mouse monoclonal antibody (Cat. # 26008).
