Plasmocin prophylactic|Invivogen ant-mpp|现货进口正品
名称 Plasmocin prophylactic
产地 Invivogen
货号 ant-mpp
规格 25 mg (5 x 2 ml)
价格 1330
保存温度 4°C or -20°C
1.产品组分：
Plasmocin is provided as a yellow solution at different concentrations:
25 mg/ml (Plasmocin Treatment)
2.5 mg/ml (Plasmocin Prophylactic)
2.保存温度：
Plasmocin Prophylactic is shipped at room temperature and should be stored at 4°C or -20°C.
Plasmocin Prophylactic is stable 6 months at 4°C and at least 2 years at -20°C. Avoid repeated freeze-thaw cycles.
注意: Presence of crystals does not alter properties of the product.
Vortex the tube until the crystals disappear .
3.用途：
Plasmocin Prophylactic can be used as a routine addition in liquid media to prevent mycoplasma and related cell wall-less bacteria contamination in mammalian cell cultures.
Plasmocin Prophylactic exhibits no toxicity in eukaryotic cells.
4.方法：
To prevent mycoplasma and related cell wall-less bacteria contamination of cell cultures that have been previously tested to be contamination-free.Plasmocin Prophylactic can be used in combination with penicillin and streptomycin.
1. Use Plasmocin Prophylactic at a concentration of 5 μg/ml, that represents a 1:500 dilution of the 2.5 mg/ml stock solution in culture medium (200 ml Plasmocin Prophylactic in 100 ml culture medium).
2. Remove and replace with fresh Plasmocin Prophylactic containing medium every 3-4 days.
3.Frequently test for the presence of mycoplasma in your cell cultures. Mycoplasma can be detected using PlasmoTest, a simple and reliable cell-based assay for the detection of mycoplasma contamination.
注意: If you detect mycoplasmas in your cells, we recommend the use of Plasmocin Treatment (25 mg/ml solution) which eliminates mycoplasmas from infected cultures.
5.描述：
支原体培养细胞的污染是主要问题
两个基本研究和工业生产。多达87%的细胞系通过mycoplasma1可能被污染。支原体不能检测到视觉检测和可能不会明显影响到△细胞文化经济增长率。然而,支原体感染已被证明改变DNA,RNA和蛋白质的合成,引入染色体失常和引起宿主细胞的改变或修改等离子体细胞膜抗原。
相较于其他anti-mycoplasma化合物,Plasmocin是活跃在两个自由mycoplasmas和细胞※内的形式。这种优势是一个组件所赋予的积极的Plasmocin运输到哺乳动物细胞。在所有的动物细胞株测试日期,甚至在五次工作浓度,没有明显的副作用是观察细胞的新陈代谢。
没有抵抗,在液体培养基的mycoplasmas曾经被确认在重复的实验尝试衡量的基因突变率。因此,开发抗支原体株基本∴上消除了。
Plasmocin 也活跃在低浓度在大范围的革兰氏阳性和革兰氏阴性细菌,否则耐药的混合体链霉素和青霉素,和展览在真核细胞没有毒性。
很多细胞感染mycoplasmas已经成功地卐治疗Plasmoci,包括胚胎干细胞,hybridomas和逆转录病毒包装细胞。
6.说明：
活跃在两个自由的mycoplasmas和细胞内的形式;
没有阻力的mycoplasmas在液体培养基;
没有明显的负面影响细胞的新陈代谢;
活跃在低浓度的广泛积极和消极的细菌;
消除支原体在只要2周;
在T75治疗多达25细胞株。
Plasmocin is a well-established antimycoplasma reagent. It contains two bactericidal components strongly active against mycoplasmas that allow their elimination in only 2 weeks.
The first component acts on the protein synthesis machinery while the second acts on the DNA replication. These two specific and separate targets are found only in mycoplasmas and many other bacteria and are completely absent in eukaryotic cells.
