近日，西北农林科技大学林学院罗志斌教授的研究成果《Net cadmium flux and accumulation reveal tissue-specific oxidative stress and detoxification in Populus×canescens》在植物学领域专业杂志《Physiologia Plantarum 》(2010 IF 3.067)发表。
本文作者应用“非损伤微测技术”研究了灰□ 杨对重金属镉(Cd2+)的吸收，发现灰杨根部置于富镉环境后，根尖会呈现极强的Cd2+内流，导致镉在根部的富集。文中的非损伤微测技术实验是在旭月公司测试中心完成的。非损伤微测技术提供了植物富集重金属最为直接的实验证据。
随着●西部地区经济发展水平的不断提高及国家对农林科技的一系列扶持政策的出台，西北农林科技大学迎来了良好的发展契机，亟需像非损伤微测技术 这样的先进技术手段来提升自身科¤研实力。除罗志斌教授外，西北＠农林科技大学还有不少学者已经在利用非损伤微测技术开展科研工作，相信不久后会陆续有高水平 研究成果诞生。
Net cadmium flux and accumulation reveal tissue-specific oxidative stressand detoxification in Populus×canescens 灰杨镉离子的流入与富集揭示了组织Ψ 特异性的氧化应激与解毒机制
Abstract:
To characterize thedynamics of Cd2+flux in the rhizosphere and to study cadmium (Cd)plant-internal partitioning in roots, wood, bark and leaves in relation toenergy metabolism, reactive oxygen species (ROS) formation and antioxidants,Populus × canescens plantlets were exposed to either 0 or 50 μMCdSO4 for up to 20 days in the nutrient solution. A strong net Cd2+ influxin root apex was observed after Cd exposure for 24 h, even if net Cd2+ influxdecreased gradually in roots. A large amount of Cd was accumulated in roots. Cdions were uploaded via the xylem to leaves and further transported to thephloemwhere significant accumulationwas detected. Cd accumulation led todecreased photosynthetic carbon assimilation but not to the depletion in solublecarbohydrates. Increased levels of ROS were present in all tissues, except thebark of Cd-exposed poplars. To combat Cd-induced superoxide and hydrogenperoxide, P. × canescens appeared to rely mainly on the formation ofsoluble phenolics as these compounds showed the highest accumulation in thebark and the lowest in wood. Other potential radical scavengers such asproline, sugar alcohols and antioxidant enzymes showed tissue- and exposuretime-specific responses to Cd. These results indicate a complex pattern ofinternal Cd allocation in P. × canescens resulting in higher ROSstress in wood than in bark and intermediate responses in roots and leaves,probably because of differential capacities of these tissues for the productionof protective phenolic compounds.
