Corning? Matrigel? hESC-Qualified Matrix
PRODUCT: BD Matrigel? hESC-qualified Matrix, 5 ml vial
CATALOG NUMBER: 354277
BD Matrigel?基质是从EngelbrethHolm -Swarm（EHS）小鼠肿瘤中提取的一种液体基质膜，室温下，可形成一种重组基质膜。BD基质胶的主要组分为层粘连蛋白， IV型胶原蛋白，巢蛋白和硫酸乙酰肝素蛋白多糖。生长因子，胶原酶，以及其他未定义的成分也存在于BD基质胶中。
BD基质胶可以作为一种基质，与各种条件确定的培养基ζ一起已经被广泛地用于培养人胚胎干细胞（hES）。对于胚胎干细胞研究，理想环境由两部分组成：hES细胞特异性的细胞培养表面和无血清的确定的培养基。
BD Matrigel hESC-qualified matrix和STEMCELL技术mTeSR（根据WiCell研究所授权开发） ，高品质的表面和培养基组合形成了一个完整的环境，以支持胚胎干细胞的伸展。BD Matrigel hESC-qualified matrix是一种优化的表面用于干细胞研究。
BD基质胶已被认定为与mTeSR1兼容，提供可重复性和一致性，对于胚胎干细※胞研究是必不可少的。
BACKGROUND:
Basement membranes are continuous sheets of specialized extracellular matrix that are found at the dermal-epidermal junction, at the base of all lumen-lining epithelia throughout the digestive, respiratory, reproductive and urinary tracts and that underlie parenchyma of endocrine and exocrine glands.
BD Matrigel? Matrix is a soluble basement membrane extract of the Engelbreth-Holm-Swarm （EHS） tumor that gels at room temperature to form a genuine reconstituted basement membrane.1 The major components of Matrigel Matrix are laminin, collagen IV, entactin and heparan sulfate proteoglycan.2-3 Growth factors, collagenases, plasminogen activators and other undefined components have also been reported in Matrigel Matrix.4-5
STEM CELLS:
Historically, hES cell derivation and culturing techniques utilized serum and/or mouse embryonic fibroblast （MEF） feeder layers.6 An ideal environment for hES cell research consists of both a cell culture surface specifically qualified for hES cells, and a serum-free, defined medium. BD Matrigel? hESC-qualified Matrix and STEMCELL Technologies’ mTeSR?1 （developed under license from the WiCell Research Institute7）, a high quality surface and medium combination, create the first complete environment to support feeder-independent expansion of hES cells.
BD Matrigel hESC-qualified Matrix is an optimized surface for your stem cell research. It has been qualified as mTeSR?1-compatible by STEMCELL Technologies, eliminating the need for time-consuming screening, while providing the reproducibility and consistency essential for your hES cell research. When coupled with a variety of culture media, Matrigel Matrix has been widely accepted as an alternative substrate for the culture of hES cells.8-9 The mTeSR?1 formulation is defined and serum-free, and has been designed to maintain and expand hES cells in an undifferentiated state when used with Matrigel Matrix as a substrate. It does not require any further addition of growth factors or supplements.
The mTeSR?1 formulation and Matrigel Matrix have been shown to be a successful combination for culturing different hES cell lines for up to 20 passages.10 Cells maintained in mTeSR?1 express high levels of pluripotency markers such as Oct-3/4 and SSEA-3, and pluripotency of cells maintained in mTeSR?1 has also been demonstrated by the ability of these cells to differentiate into all three germ layers in the teratoma assay.7,11
SOURCE: Engelbreth-Holm-Swarm （EHS） Mouse Tumor
FORMULATION: Dulbecco's Modified Eagle's Medium with 50 μg/ml gentamycin
Matrigel hESC-qualified Matrix is compatible with all culture media
STABILITY: Stable for a minimum of three months from day of shipment when stored at -20°C
Stable for a minimum of six months when stored in aliquots at -70°C
RECONSTITUTION AND USE:
Color variations may occur in frozen or thawed vials of BD Matrigel hESC-qualified Matrix, ranging from straw yellow to dark red due to the interaction of carbon dioxide with the bicarbonate buffer and phenol red. This is normal, does not affect product efficacy, and will disappear upon equilibration with 5% CO2.
Thaw Matrigel on ice. Once thawed, swirl the vial to ensure the Matrigel is evenly dispersed. Spray top of vial with 70% ethanol and air dry. Keep product on ice and handle using sterile technique. Dispense material into appropriate aliquots, using pre-cooled tubes, and refreeze immediately. Avoid multiple freeze thaws. DO NOT STORE IN FROST-FREE FREEZER.
CAUTION:
BD Matrigel hESC-qualified Matrix will gel rapidly at 22oC to 35oC. Thaw overnight at 4oC on ice. Gelled Matrigel may be re-liquified if placed at 4°C on ice for 24-48 hours.
MatrigelTM选择
?低生长因子（GFR）的Matrigel 基底膜基质适用于对基质成分要求严格的实验，如细胞信号通路和细胞因子的研究等
?不含酚红的Matrigel适用于荧光检测或显色反应等分析
?高浓度的Matrigel适用于研究血管生成、肿瘤细胞迁移和体内肿瘤模型的建立等
