P-9005-48EpiQuik m6A RNA Methylation Quantification Kit(Colorimetric)
P-9005-96EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
品牌：Epigentek
规格：48T/96T
用途: The EpiQuik? m6A RNA Methylation Quantification Kit (Colorimetric) is suitable for detecting N6-methyladenosine (m6A) RNA methylation status directly using total RNA isolated from any speciessuch as mammals, plants, fungi, bacteria, and viruses.
Input RNA: The amount of RNA for each assay can be 100 ng to 300 ng. For optimal quantification,the input RNA amount should be 200 ng, as the abundance of m6A is generally less than 0.1% of total RNA.
Starting Material: Starting materials can include various tissue or cell samples such as cells from flaskor microplate cultured cells, fresh and frozen tissues, paraffin-embedded tissues, blood, body fluid samples, etc.
Internal Control: Both negative and positive RNA controls are provided in this kit. A standard curve can be performed (range: 0.02 to 1 ng of m6A) or a single quantity of m6A can be used as a positive control. Because m6A content can vary from tissue to tissue, and from normal and diseased states, or vary under treated and untreated conditions, it is advised to run replicate samples to ensure that the signal generated is validated. This kit will allow the user to quantify an absolute amount of m6A and determine the relative m6A RNA methylation states of two different RNA samples.
Precautions: To avoid cross-contamination, carefully pipette the sample or solution into the strip wells. Use aerosol-barrier pipette tips and always change pipette tips between liquid transfers. Wear gloves throughout the entire procedure. In case of contact between gloves and sample, change gloves immediately.
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