HiTrap Q HP, 5 x 1 ml
HiTrap Q HP are prepacked, ready-to-use Q Sepharose High Performance strong anion exchange columns for high-resolution, small-scale protein purification.
34 μm bead size for high-performance, high-resolution purifications.
Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series.
Designed for use with a syringe, peristaltic pump, and chromatography systems such as ?KTA design.
Strong quaternary ammonium (Q) anion exchanger.
The small particle size (34μm) of the medium allows fast adsorption and desorption even at high sample loadings and flow rates.SP Sepharose High Performance is a strong cation exchange medium and Q Sepharose High Performance is a strong anion exchange medium. Both remain charged and have high loading capacities over broad pH ranges.
HiTrap Q HP, 5 x 1 ml
Bed Dimensions | 7 mm × N/A |
Bed Volume | 1 ml |
Storage Conditions | 4 to 30°C, 20% Ethanol |
Pressure Max. [Over the Packed Bed During Operation] | N/A (N/A) |
Media | |
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BioProcess resin | Yes |
Ligand | Quaternary amine |
Particle size, d50V | 34 μm1) |
Ion Exchanger Type | Strong anion exchanger |
Matrix | 6% cross-linked agarose |
pH Stability Working Range | 2–12 |
pH stability, CIP | 1–14 |
pH Stability Cleaning | 1–14 |
Pressure/Flow Specification | 150 cm/h (at < 3 bar, 25°C, 10 cm bed height) |
Exclusion Limit [Mr] [Globular Proteins] | 4 x 106 |
Storage Conditions | 4 to 30°C, 20% Ethanol |
Chemical Stability | Stable in all commonly used buffers: 8 M urea, 6 M guanidine HCl, 70% ethanol, 1 M NaOH, 1 M acetic acid, 30% isopropanol, 30% acetonitrile, 2% SDS 2) |
Charged Groups | - N+(CH3)3 |
1)d50vis the median particle size of the cumulative volume distribution. | |
2)1 M NaOH and acetic acid should be used only for cleaning purposes. |
Column | |
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Column i.d. | 7 mm |
Bed Dimensions | 7 mm × N/A |
Complete Packsize | 1 ml |
Material [Column Hardware] | Polypropylene (PP) |