PD-10 desalting columns packed with Sephadex G-25 resin
PD-10 Desalting Columns contain Sephadex G-25 resin for rapid buffer exchange, desalting, and removal of small contaminants (salts, dyes, radioactive labels) from samples using gravity flow or centrifugation.
PD-10 Desalting Columns contain Sephadex G-25 resin for rapid buffer exchange, desalting, and removal of small contaminants (salts, dyes, radioactive labels) from samples using gravity flow or centrifugation.
Flexible run protocols– perform desalting and buffer exchange using gravity flow (1.0 to 2.5 mL samples) or centrifugation (1.75 to 2.5 mL samples)
Flexible throughput– desalt one sample or multiple samples in parallel
Good recovery– in the range of 70% to > 95%
High desalting capacity– remove > 98% salt with gravity; > 90% with centrifugation
Minimal dilution– typically none when used as spin columns; 1.4× when used with gravity flow
Time savings compared to dialysis– inexpensive and convenient alternative to lengthy and troublesome dialysis procedures
Parameter | Disposable PD-10 Desalting Column, with Sephadex G-25 resin, 1.0–2.5 mL samples |
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Bed dimensions | 14.5 × 50 mm |
Bed height | 50 mm |
Bed volume | 8.3 |
Column i.d. | 14.5 mm |
Includes | 1 × Column stand 4 × PD-10 spin adaptor 1 × Buffer tray 30 × Bottom sleeve Instructions are supplied with the columns |
Order Information | Disposable columns for clean-up of proteins/oligosaccharides. The columns are prepacked with Sephadex G-25 resin and for sample volumes up to 2.5 mL by gravity flow and columns spin flow. PD- 10 Buffer reservoir has to be ordered separately For smaller scale please use PD MidiTrap G-25 (1.0 mL), PD MiniTrap G-25 (0.5 mL), PD SpinTrap G-25 (130 μL) and PD MultiTrap G-25 (130 μL) in parallel |
Sample volume | 2.5 ml |
Storage | 4 to 30°C, 0.15% Kathon CG/ICP Biocide |
Wetted Part | No |
Pack size | 30 columns |