Epoxy-Activated Sepharose 6B
Epoxy-activated Sepharose 6B is a pre-activated resin for immobilization of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose 6B via a 12-atom hydrophilic spacer arm
Epoxy-activated Sepharose 6B is a pre-activated resin for immobilization of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose 6B via a 12-atom hydrophilic spacer arm
Epoxy-activated Sepharose 6B can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups.
Epoxy-activated Sepharose 6B is a pre-activated resin for immobilization of various ligands. Epoxy-activated Sepharose 6B can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups. Other ligands can be coupled through hydroxyl, amino or thiol groups. The resin has a long hydrophilic spacer arm which makes it particular suitable for immobilization of small molecules. Epoxy-activated Sepharose 6B is formed by reacting Sepharose 6B with 1,4-bis (2,3- epoxy-propoxy-) butane.
Parameter | Epoxy-Activated Sepharose 6B, 15 g |
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Chromatography technique | Affinity coupling |
Particle size, d50V1 | ~90 μm |
Group To be Coupled | -NH?, -OH, or -SH |
Matrix | Agarose, 6% |
Particle Size | 45 μm-165 μm |
Application | The resin has a long hydrophilic spacer arm which makes it particular suitable for immobilization of small molecules |
Pressure/Flow Specification | base matrix 100-200 cm/h, pressure drop cm H?O/bed height=15, bed height 10 cm, 5 cm i.d. |
Flow velocity | < 75 cm/h at 25 °C, HR 16/10 column, 5 cm bed height |
Storage | 2 to 8°C |
Chemical stability | Stable to commonly used aqueous solutions. Can be used with non-ionic detergents, denaturing solvents, e.g. 8 M urea and 6 M guanidine hydrochloride, Stable in organic solvents, e.g. 50% methylformamide and 50% dioxane. |
Pack size | 15 g |