Superose 6 Increase small-scale SEC columns
Size exclusion chromatography columns: for high-resolution protein purification and analysis in MW range Mr~ 5K to ~ 5M.
Superose 6 Increase SEC columns are designed for high-resolution, small-scale preparative purification, and analysis of large globular proteins, protein complexes, and other macromolecules in the molecular weight range Mr~5K to ~5M.
Wide fractionation range: optimized to purify large biomolecules, including membrane proteins, protein complexes, and DNA fragments
Very high resolution: small bead size and a narrow particle size distribution provide high resolution, for high protein purity
High flow rates: rigid beads give excellent pressure/flow properties
Enhanced performance: improved resolution and runtime compared to predecessor
“Increase”: Cytiva's new generation of size exclusion chromatography columns
Superose 6 Increase is a next-generation, agarose-based matrix for size exclusion chromatography. With smaller and more rigid beads than their predecessors, Superose 6 Increase columns deliver higher resolution protein purification in shorter runtimes.
Parameter | Superose 6 Increase 10/300 GL |
---|---|
Bed dimensions | 10 x 300 |
Bed height | 300 to 310 mm |
Bed support | Filter |
Bed volume | ~ 24 mL |
Chemical stability | Conditions where the resin may be operated without significant change in function: ? All commonly used aqueous buffers, pH 3 to 12 ? Urea, up to 8 M ? Ionic and nonionic detergents, e.g., 1% SDS ? Guanidine hydrochloride, up to 6 M ? Isopropanol, up to 5% ? Methanol, up to 10% ? Sodium hydroxide, up to 0.5 M ? Dithiothreitol, up to 5 mM Conditions where the resin can be subjected to cleaning- or sanitization-in-place without significant change in function: ? Acetonitrile, up to 30% ? Sodium hydroxide, up to 1 M ? Ethanol, up to 70% ? Acetic acid, up to 1 M ? Isopropanol, up to 30% ? Hydrochloric acid, up to 0.1 M ? Trifluoroacetic acid, up to 10% ? Formic acid, up to 70% Avoid: ? Oxidizing agents ? Unfiltered solutions |
Chemical stability (column hardware) | Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons |
Column i.d. | 10 mm |
Exclusion limit (Mr) (globular proteins) | ~ 4 × 107 |
Fractionation range (Mr) (globular proteins) | 5000 to 5 × 106 |
Material (column hardware) | Borosilicate glass, polyetheretherketone (PEEK), polypropylene (PP), ethylene propylene diene monomer (EPDM), and perfluoro-rubber (PFR) |
Material (bed support) | Polyethylene (PE) filter |
Material (column tube) | Borosilicate glass |
Matrix | Composite of cross-linked agarose |
Operating flow rate, max. | 1.50 mL/min, water at room temperature; 0.75 mL/min, 20% ethanol at room temperature; 0.75 mL/min water at low temperature; 0.35 mL/min 20% ethanol at low temperature |
Operating flow rate, recommended1 | 0.50 mL/min |
Particle size, d50V2 | ~ 8.6 μm |
Sample volume | 25 to 500 μL |
Storage conditions | 4?C to 30?C, 20% ethanol |
Theoretical plates | > 48 000 m-1 |
Typical pressure drop over the packed bed3 | ~ 3 MPa |
pH stability, cleaning-in-place (CIP) | 1 to 14 |
pH stability, operational | 3 to 12 |
1Water at 20?C to 25?C.
2Median particle size of the cumulative volume distribution.
3At 25?C in water. Determine the individual column limit according to product instructions for use.