Our Cell Explorer? fluorescence imaging kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions. The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context. This particular kit is designed to uniformly label live cells for the flow cytometric analysis of live cells with the violet laser (405 nm excitation). The kit uses a proprietary dye that gets enhanced fluorescence upon entering into live cells. The dye is a hydrophobic compound that easily permeates intact live cells. The hydrolysis of the weakly fluorescent substrate by intracellular esterases generates a strongly fluorescent hydrophilic product that is well-retained in the cell cytoplasm. It can be readily adapted for flow cytometry applications. The fluorescent dye used in the kit is well excited with the violet laser (405 nm excitation) to fluorescence at ~550 nm. The kit provides all the essential components with an optimized cell-labeling protocol. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity.
Platform
Flow cytometer | |
Excitation | 405 nm laser |
Emission | 525/50 nm filter |
Instrument specification(s) | Pacific Orange channel |
Fluorescence microscope | |
Excitation | 405 nm |
Emission | 545/25 filter |
Recommended plate | Black wall/clear bottom |
Components
Component A: CytoCalcein? Violet 550 | 2 vials |
Component B: HHBS (Hanks' buffer with 20 mM Hepes) | 1 bottle (100 mL) |