ExoView外泌体全面表征试剂盒
外泌体计数、粒径、蛋白表达、蛋白共定位一次完成
检测样本类型 | 对细胞培养上清、血浆、血清、尿液、脑脊液、唾液等生物样本中的外泌体№直接进行分析 |
捕获抗体种类 | anti-CD81, anti-CD9, anti-CD63, 同型IgG对照；可自定义 |
单次上样体积 | 35 μl稀释样本 |
重复检测数目 | 3复孔 |
荧光抗体种类 | CD9（Blue）/ CD81（Green）/ CD63（Red） |
实验原理
① 35 μL外泌体样品滴加在芯片上孵育；
② 预先包被的抗体特异结合外泌体Ψ表面蛋白以捕获外泌体；
③ 再使用荧光抗体特异性标记需要表征的标记物；
④ 用ExoView R100检测外泌体︽粒径、计数、蛋白表达（CD9，CD81，CD63等）及共定位。
检测流程
产品类别
| 产品货号 | 产品名称 |
| EV-TETRA-C | 人外泌体检测试剂【盒 |
| EV-TETRA-P | 人血浆外泌体检测试剂盒 |
| EV-TETRA-M2 | 鼠外泌体检测试剂盒 |
| EV-TETRA-C-CAR | 人外泌体内容物¤检测试剂盒 |
| EV-TETRA-P-CAR | 人血浆外泌体内容物检测卐试剂盒 |
| EV-TC-FLEX | 自由捕获人外泌体检测试剂盒 |
| EV-TP-FLEX | 自由捕获人血浆外泌体检测试剂盒 |
| EV-TC-FLEX-CAR | 自由捕获◣人外泌体内容物检ξ 测试剂盒 |
| EV-TP-FLEX-CAR | 自由捕获人血浆外泌◆体内容物检测试剂盒 |
| EV-TM-FLEX | 自由捕获☆鼠外泌体检测试剂盒 |
| EV-TM-FLEX-CAR | 自由捕获鼠外泌体内容物检测试剂盒 |
| EV-FLEX-2 | 自由捕获外泌体检测试剂盒 |
| EV-FLEX-2 -CAR | 自由捕获ω外泌体内容物检测试剂盒 |
| EV-CTETRA-1/2/3 | 人外泌体检测试剂盒+1/2/3个自定义捕获抗∞体 |
| EV-CTETRA-1/2/3-CAR | 人外泌体内容物检测试剂盒+1/2/3个自定义捕获抗体 |
| EV-CUST-1/2/3/4/5/6 | 自定义1/2/3/4/5/6抗体捕获外泌体检测试剂ω 盒 |
| EV-CUST-1/2/3/4/5/6-CAR | 自定义1/2/3/4/5/6抗体捕获外泌体内容物检测试剂盒 |
试剂盒特点
特异性捕获 芯片上可包被多※达6种捕获抗体，特异性捕获含特定蛋白标记物的外泌体。 | |
jue对阳性外↑泌体计数 芯片捕获外】泌体后，可通过SP-IRIS技术直接检测样品中外泌体的数量。 | |
单个外泌体蛋白共定位分析 检测每」个外泌体的∴荧光信号并进行统计，可获得荧光共定位信息，用于分析样品中不同表型外泌体的比例（如『右图所示）。 | |
无需纯化 使用抗体Ψ捕获模式，防止样品中杂质影响结果，可直接检测血液、尿液和细胞培养液中的外泌体，未纯化样品的测量结果与纯△化后基本一致（如右图所示）。 | |
粒径分辨〓率高 高精度SP-IRIS技术，可检测≥50 nm的外泌体，测量结果与电子显微←镜检测结果基本一致，并统计生成外泌体的粒径分¤布结果（如右图所示）。 | |
可检测外泌体内容物 试剂盒配套◣相应的穿膜剂，可穿透外泌体并对外泌体内容物进行染色并检测，未穿膜时只能检测到跨膜蛋白CD9的荧光信号，穿膜后即可检测到外泌体内容物Syntenin的表达（如右图所示）。 |
测试数据
外泌体荧光数量统计
外泌体粒径检测
外泌体内容物检测
荧光强度与粒径关系※
荧光共定位分析
发表文章
? Andras Saftics.(2021) Data evaluation for surface-sensitive label-free methods to obtain real-time kinetic and structural information of thin films: A practical review with related software packages. Advances in Colloid and Interface Science.
? Kyoung-Won Ko.(2021) Integrated Bioactive Scaffold with Polydeoxyribonucleotide and Stem-Cell-Derived Extracellular Vesicles for Kidney Regeneration. ACS Nano.
? Tanina Arab. (2021) Characterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single‐particle analysis platforms. Journal of Extracellular Vesicles.
? Niaz Z.Khan.(2021) Spinal cord injury alters microRNA and CD81+ exosome levels in plasma extracellular nanoparticles with neuroinflammatory potential. Brain, Behavior, and Immunity.
? Dario Brambilla. (2021) EV Separation: Release of Intact Extracellular Vesicles Immunocaptured on Magnetic Particles. Analytical Chemistry.
? Enkhtuya Radna. (2021) Extracellular vesicle mediated feto-maternal HMGB1 signaling induces preterm birth. Lab on a Chip.
? Li, M., Soder. (2021) WJMSC‐derived small extracellular vesicle enhance T cell suppression through PD‐L1. Journal of Extracellular Vesicles.
? Crescitelli, R. (2021) Isolation and characterization of extracellular vesicle subpopulations from tissues. Nature protocols.
? Berger, A. (2021). Local administration of stem cell-derived extracellular vesicles in a thermoresponsive hydrogel promotes a pro-healing effect in a rat model of colo-cutaneous post-surgical fistula. Nanoscale.
? Vidal, M. (2020) Exosomes and GPI-anchored proteins: Judicious pairs for investigating biomarkers from body fluids. Advanced drug delivery reviews.
? K Cho, H Kook.(2020)Study of immune-tolerized cell lines and extracellular vesicles inductive environment promoting continuous expression and secretion of HLA-G from semiallograft immune tolerance during pregnancy. Journal of Extracellular Vesicles.
? Maximillian A. Rogers.(2020)Annexin A1–dependent tethering promotes extracellular vesicle aggregation revealed with single–extracellular vesicle analysis. Cell Biology.
? Annette M. Marleau.(2020)Targeting tumor-derived exosomes using a lectin affinity hemofiltration device. Cancer Research.
? Alessandro Gori.(2020)Membrane-Binding Peptides for Extracellular Vesicles On-Chip Analysis.Journal of Extracellular Vesicles.
? Rossella Crescitelli.(2020)Subpopulations of extracellular vesicles from human metastatic melanoma tissue identified by quantitative proteomics after optimized isolation. Journal of Extracellular Vesicles.
? Maria S. Panagopoulou.(2020) Phenotypic analysis of extracellular vesicles: a review on the applications of fluorescence. Journal of Extracellular Vesicles.
? WeiYan.(2020) Immune Cell-Derived Exosomes in the Cancer-Immunity Cycle. Trends in Cancer.
? Daniel Bachurski. (2019) Small RNA Sequencing across Diverse Biofluids Identifies Optimal Methods for exRNA Isolation. Cell.
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