描述
The MEGAscript? T7 Kit is an ultra-high yield in vitrotranscription kit. The high yields are achieved by modifying typical transcription reaction conditions so that very high nucleotide concentrations can be effectively used. The T7 Enzyme Mix and the 10X Reaction Buffer are specifically calibrated for each lot and RNA polymerase.Using the MEGAscript? T7 KitThe MEGAscript? T7 Kit contains in vitrotranscription reaction components and a control template. The kit will yield a total of 3–5 mg of RNA (approximately 100 μg of RNA or more per reaction) from the control template supplied with the kit. This corresponds to 400–650 moles of RNA for each mole of template. Smaller templates typically yield a lower mass and a higher molar yield of product. Novel transcription reaction conditions and a patented, high-yield technology allow the synthesis of 10–50 times the amount of RNA produced by conventional transcription reactions.ApplicationsThe MEGAscript? T7 Kit is intended for the synthesis of large amounts of unlabeled or low specific activity RNA for a variety of uses, including in vitrotranslation, antisense/microinjection studies, and isolation of RNA binding proteins. In large-scale transcription reactions, the concentration of all 4 nucleotides is high, well above the Kmfor the enzyme. The MEGAscript? T7 Kit typically yields over 10 times more RNA than conventional in vitrotranscription reactions. The kit is not recommended for synthesis of high specific activity probes.Accessory productsMessageAmp? aRNA amplification kits are powered by MEGAscript? technology and have been developed especially for the amplification of cRNA prior to array analysis. To purify in vitrotranscription products from free nucleotides, buffer components, and enzymes, Life Technologies recommends using the MEGAclear? Kit (Cat. No. AM1908) and the MEGAclear?-96 Kit (Cat. No. AM1909).
规格
| 产品类型: | In Vitro Transcribed RNA |
|---|---|
| 适用于（应用）: | In Vitro Transcription |
| 产品规格: | Kit |
| 产品类型: | Kit |
| 数量: | 25 reactions |
| 产量: | 100 μg |
| 促进剂: | T7 |
| 运输条件: | Dry Ice |
| 原始材料: | PCR Products, Linearized DNA |
| 产品线: | Ambion?, MEGAscript? |
内容及储存
T7 Enzyme Mix, 10X Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.
