大视野单分子超分辨模块 |
abbelight SAFe180是一款基于单分子定位技术的显微成像（SMLM）的超分辨模块。该设备具有高度灵活性，能够搭载在绝大多数的倒ξ置显微镜上，并且仅仅需要使用一个C-mount（CCD或CMOS所连接的部位）接口，即可将您的倒置显微镜直接升级为超分辨成像ξ 系统。并且改造过程不会破坏原有显微镜系统的光路和功能，不会与其它的『显微镜改造相冲突。
本设备既在配置上的选择也十分灵活。它既可以作为显微镜的一个升级配件来改造您的显微镜，也拥有完整的超∏分辨系统。让用户在获得专业的图像质量的同时，获得经济合理的超分辨升级方案。
大视野单分子超分辨模块-SAFe 180
设备参数
+ 模块化系统：可接到↓大多数倒置荧光显微镜
+ 成像模式：PALM、STORM、smFRET、PAINT、SPT
+ 光源模式：Epi、TIRF、HILO
+ 超高分辨率：25 nm的XY轴分辨率，50nm的Z轴分辨率
+ 超大视野：180 × 180 μm2的视野
+ 全自动化控制
+ 无需高功率激光光源
| 加装 | TIRF PALM STORM SPT smFRET ...... | 兼容 | Confocal Spinning-Desk Widefield SIM STED |
Now We See......
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| 超大的视野神经元 | 伪足小体 |
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| 模式生物 | 微管蛋白 |
配套试剂
| Smartkit | Compatibledyes |
- 10 doses per box - 200 μL per dose - 30 sec prepartion - 2 months in a fridge - 2 weeks on sample | - Atto 488, WGA-AF?488 - AF?532, CF?532, Cy3b - AF?555, AF?594, CF?555, AF?568, CF?568, Cy5, MemBriteTM 568, TMR - AF?647, CF?647, AF?680, CF?680, MemBriteTM 640, Actin-stain 670, SiR647 |
发表文◇献列表
[1] Cabriel, Clément, et al. "Combining 3D single molecule localization strategies for reproducible bioimaging." Nature communications 10.1 (2019): 1980.
[2] Woodhams, Stephen G., et al. "Cell type–specific super-resolution imaging reveals an increase in calcium-permeable AMPA receptors at spinal peptidergic terminals as an anatomical correlate of inflammatory pain." Pain 160.11 (2019): 2641-2650.
[3] Belkahla, Hanen, et al. "Carbon dots, a powerful non-toxic support for bioimaging by fluorescence nanoscopy and eradication of bacteria by photothermia." Nanoscale Advances (2019).
[4] Denis, Kevin, et al. "Targeting Type IV pili as an antivirulence strategy against invasive meningococcal disease." Nature microbiology 4.6 (2019): 972.
[5] Szabo, Quentin, et al. "TADs are 3D structural units of higher-order chromosome organization in Drosophila." Science advances 4.2 (2018): eaar8082.
[6] Boudjemaa, Rym, et al. "Impact of bacterial membrane fatty acid composition on the failure of daptomycin to kill Staphylococcus aureus." Antimicrobial agents and chemotherapy 62.7 (2018): e00023-18.
[7] Culley, Sian, et al. "Quantitative mapping and minimization of super-resolution optical imaging artifacts." Nature methods 15.4 (2018): 263.
[8] Berger, Stephen L., et al. "Localized myosin II activity regulates assembly and plasticity of the axon initial segment." Neuron 97.3 (2018): 555-570.
[9] Cabriel, Clément, et al. "Aberration-accounting calibration for 3D single-molecule localization microscopy." Optics letters 43.2 (2018): 174-177.
[10] Bouissou, Ana?s, et al. "Podosome force generation machinery: a local balance between protrusion at the core and traction at the ring." ACS nano 11.4 (2017): 4028-4040.
[11] Sellés, Julien, et al. "Nuclear pore complex plasticity during developmental process as revealed by super-resolution microscopy." Scientific reports 7.1 (2017): 14732.
[12] Bourg, Nicolas, et al. "Direct optical nanoscopy with axially localized detection." Nature Photonics 9.9 (2015): 587.
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