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                3D单分@子荧光成像系统-SAFe 360

                3D单分子荧光⊙成像系统-SAFe 360
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                • 3D单分■子荧光成像系统-SAFe 360
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                abbelight
                SAFe 360
                abbelight
                高教
                法国
                详细说明

                3D单分子荧光成█像系统-SAFe 360

                3D单分【子荧光成像系统-SAFe 360

                  SAFe 360是法国abbelight公司推出的一款基于单分子定位的显微成像(SMLM)的新型3D单分子成像系统,它独有的DAISY技术整合了散光技术和超临界角光技术,能◢够极大的提高定位精度,xyz三轴定位精度高达15nm,可以提供高清晰三维亚细胞结构图像,支持同时多四色成像,可以用于细胞纳米三维成】像,观测高清晰亚细胞器结构,实时研究不同的结构功能蛋白的№共定位信息,在单分子水平研究分子动力学反应以及细胞间的相互作用╳等。

                3D单分子荧光成像系统-SAFe 360

                3D单分子荧光成像系统-SAFe 360

                  

                设备参数

                  +  成像模式:PALM、STORM、PAINT、smFRET 、SPT

                  +  光源模式:Epi、TIRF、HILO

                  +  超高分辨率:15 nm的XYZ轴分辨率

                  +  超大视野:200 × 200 μm2的视野

                  +  一次可同时采集1.2 μm深度图像♀信息

                  +  最大图像深度:10 μm

                  +  实时漂移矫正

                  +  四色同时成像

                  +  活细胞成像模式

                加装TIRF

                PALM

                STORM

                SPT

                smFRET

                ...... 

                兼容Confocal

                Spinning-Desk

                Widefield

                SIM

                STED

                3D单分子荧光成像系统-SAFe 360

                  

                Now We See......

                3D单分子荧光成像系统-SAFe 360

                3D单分子荧光成像系统-SAFe 360

                3D线粒体结构核孔复合物

                3D单分子荧光成像系统-SAFe 360

                3D单分子荧光成像系统-SAFe 360

                老鼠海马神经元微管蛋白网络

                  

                配套试剂

                SmartkitCompatibledyes

                - 10 doses per box

                - 200 μL per dose

                - 30 sec prepartion

                - 2 months in a fridge

                - 2 weeks on sample

                - Atto 488, WGA-AF?488

                - AF?532, CF?532, Cy3b

                - AF?555, AF?594, CF?555, AF?568, CF?568, Cy5, MemBriteTM 568, TMR

                - AF?647, CF?647, AF?680, CF?680, MemBriteTM 640, Actin-stain 670, SiR647

                  

                发表」文献列表

                  [1] Radhakrishnan, A. V., et al. "Single-Protein Tracking to Study Protein Interactions During Integrin-Based Migration." The Integrin Interactome. Humana, New York, NY, (2021). 85-113.

                  [2] Jouchet, Pierre, et al. "Nanometric axial localization of single fluorescent molecules with modulated excitation." Nature Photonics(2021): 1-8.

                  [3] Pernier, Julien, et al. "Myosin 1b flattens and prunes branched actin filaments." Journal of cell science133.18 (2020).

                  [4] Jimenez, Angélique, Karoline Friedl, and Christophe Leterrier. "About samples, giving examples: optimized single molecule localization microscopy." Methods 174 (2020): 100-114.

                  [5] Mau, Adrien, et al. "Fast scanned widefield scheme provides tunable and uniform illumination for optimized SMLM on large fields of view." bioRxiv(2020).

                  [6] Orre, Thomas, et al. "Molecular motion and tridimensional nanoscale localization of kindlin control integrin activation in focal adhesions." bioRxiv(2020).

                  [7] Cabriel, Clément, et al. "Combining 3D single molecule localization strategies for reproducible bioimaging." Nature communications10.1 (2019): 1980.

                  [8] Woodhams, Stephen G., et al. "Cell type–specific super-resolution imaging reveals an increase in calcium-permeable AMPA receptors at spinal peptidergic terminals as an anatomical correlate of inflammatory pain." Pain160.11 (2019): 2641-2650.

                  [9] Belkahla, Hanen, et al. "Carbon dots, a powerful non-toxic support for bioimaging by fluorescence nanoscopy and eradication of bacteria by photothermia." Nanoscale Advances(2019).

                  [10] Denis, Kevin, et al. "Targeting Type IV pili as an antivirulence strategy against invasive meningococcal disease." Nature microbiology4.6 (2019): 972.

                  [11] Szabo, Quentin, et al. "TADs are 3D structural units of higher-order chromosome organization in Drosophila." Science advances4.2 (2018): eaar8082. 

                  [12] Boudjemaa, Rym, et al. "Impact of bacterial membrane fatty acid composition on the failure of daptomycin to kill Staphylococcus aureus." Antimicrobial agents and chemotherapy62.7 (2018): e00023-18.

                  [13] Culley, Sian, et al. "Quantitative mapping and minimization of super-resolution optical imaging artifacts." Nature methods15.4 (2018): 263.

                  [14] Berger, Stephen L., et al. "Localized myosin II activity regulates assembly and plasticity of the axon initial segment." Neuron97.3 (2018): 555-570.

                  [15] Cabriel, Clément, et al. "Aberration-accounting calibration for 3D single-molecule localization microscopy." Optics letters43.2 (2018): 174-177. 

                  [16] Bouissou, Ana?s, et al. "Podosome force generation machinery: a local balance between protrusion at the core and traction at the ring." ACS nano11.4 (2017): 4028-4040. 

                  [17] Sellés, Julien, et al. "Nuclear pore complex plasticity during developmental process as revealed by super-resolution microscopy." Scientific reports7.1 (2017): 14732.

                  [18] Bourg, Nicolas, et al. "Direct optical nanoscopy with axially localized detection." Nature Photonics9.9 (2015): 587. 

                  

                用户单位

                3D单分子荧光成像系统-SAFe 360

                3D单分子荧光成像系统-SAFe 360

                3D单分子荧光成像系统-SAFe 360

                3D单分子荧光成像系统-SAFe 360

                3D单分子荧光成像系统-SAFe 360

                3D单分子荧光成像系统-SAFe 360

                3D单分子荧光成像系统-SAFe 360

                3D单分子荧光成像系统-SAFe 360


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