StemRDPSGro人iPS细胞和胚胎干细胞无血清培养基
StemRD PGroF-500 PSGro-case, Human iPSC/ESC growth medium 人iPSC/ESC胚胎干细胞无血清培养基 500mL/套
产品英文名称：StemRD PGroF-500 PSGro-case, Human iPSC/ESC Growth Medium
产品中文名称：StemRD 人iPS细胞和胚胎干细胞无血清培养基,无异源级别
StemRD PGroF-500 PSGro-case, Human iPSC/ESC growth medium 人iPSC/ESC胚胎干细胞无血清培养基产品特点：
★ Serum-Free 无血清成分；
★ Xeno-Free 无异种蛋白；
★ Chemically-Defined 化学成分明确；
★ Feeder-Independent 无需滋养层细胞；
★ Supports human iPSCs/ESCs 用于人iPS细胞和胚胎干细胞生长；
★ Performs Comparably to mTeSR 1 效能与mTeSR 1培养基同等。
Characteristic Morphology
Human ESCs(A,B); iPSCs(C,D) in PSGro-Free®
PSGro-Free 是stemRD开发的一款①针对人类胚胎干细胞
（ESCs）和多能诱导干细胞（iPSCs）的生长ㄨ维护培养
基。我们生产质量可靠的生长因子，并拥有来自System
Biosciences的优秀iPS研究专家。PSGro-Free的所有添
加组分都为化学合成或↘已纯化的重组蛋白，并且决不含有
任♂何非人源的异种成分。
PSGro-Free is a growth medium designed for human
induced pluripotent stem cells ( iPSCs ) and human
embryonic stem cells (ESCs). It was developed at
StemRD, a premier producer of growth factors, in
collaboration with iPS experts at System Biosciences.
红荣微再（上海）生』物工程技术有限公司主营：干细胞、精准医疗、细胞治疗、器官再生四大板块的产品，以“传递科学价值，服务科学研究※”为宗旨，经过近两年的㊣ 努力推广，红荣微再（上海）生物工→程技术有限公司在全中国代理的CellArtis干细胞产品已经成功销往：上海市、北京市、重庆市、天津市、广东省的广州市、深圳市、珠海市、江西省的南昌市、四川省的成都市∑、绵阳、浙江省的杭】州市、宁波、温州、江苏省的南京市、苏州、无锡、泰州、湖南省的长沙、陕西省的西安市、杨凌、甘肃省的兰〖州、宁夏的银川〓、新疆的乌鲁木齐←、广西省的南＠ 宁市、山东省的济南、青岛、东北三省辽宁省的〗沈阳市、大连市、吉林省的长◥春市、黑龙江省■的哈尔滨市等中国绝大部分省市和自治区【。
欢〗迎您致电╲ 华雅再生医←学旗舰公司：红荣微再（上海）生物工程技术有限①公司
StemRD PGroF-500 PSGro-case, Human iPSC/ESC growth medium 人iPSC/ESC胚胎干细胞无血清培养基 产品英文描述如下：
PSGro-caseis designed for human induced pluripotent stem cells (iPSCs) and human embryonic stem cells (ESCs). It is serum-free and chemically-defined as all of its ingredients are synthetic chemicals or purified recombinant proteins. PSGro-casesupports feeder-free growth and maintenance of human iPSCs or human ESCs on extra-cellular matrix proteins (e.g., BD Matrigel?). Its performance is comparable to or better than the market-leading mTeSR?1 or E8 in growth rate, characteristic morphology, pluoripotent markers, normal karyotype, and differentiation to different germ layers. The robustness and stability of PSGro-caseenable seeding cells over the weekend without changing medium, so you have the weekend Free.
StemRD PGroF-500 PSGro-case, Human iPSC/ESC Growth Medium 产品组分：
|
product |
Catalog No |
size |
price |
|
PSGro kit 500ml |
PGro-500 |
500ml |
¥2587 |
|
PSGro case of 12 kits |
PGro-case |
12X500ml |
¥25987 |
提供产品：华雅再︻生医学旗舰公司-红荣微再（上海）生物▓工程技术有限公司为中国再生①医学和生物工程技术领域的组织器官再生研究、组织器官功能重建研∮究、干细胞技术研↑究、组织工程』生物支架材料研究、器官移植医疗器械产品等再生医学产业链的相关客户提供：iPS/ES胚胎干细胞的→种子细胞、心肌细胞、人源肝脏细胞、MSC间充质干细●胞、基底膜基质胶细胞支架、干细胞生长分化调控因子、干细胞药物研究的小分子抑▅制剂、调节剂、小分子▲化合物、功能⊙胶原蛋白生物材料、3D细胞︾培养基质胶、器官模型3D打印技术材料、神经干细胞培养基、STEM121抗体、BD科研P100、P800蛋白『质组学采血管、BD血浆准备㊣ 管PPT管、BD PAXgene全血RNA样本收ξ集管、BD公司CPT/PRP美容々采血管、StreckCell-Free RNA BCT游离RNA样本□收集管、StreckCell-Free DNA Urine Preserve游离DNA尿液保ω 存管、StreckCell-Free DNA BCT游离DNA采血管、循环肿★瘤细胞CTC及无▓创产前基因检测采血管、illumina高品质NGS文库制备△试剂盒、ThruPLEX ctDNA测序试剂盒、全基因组测序、全外显子测▽序、靶向测序、ChIP-Seq测序、CNV和染色体○异倍性分析、Rubicon Genomics单细〓胞全基因组测序试剂盒、生物医学试剂耗材仪器等种□类丰富的产品，以“Focus on Tissue and Organ Regeneration专注组织器官再生与功能重建”为己任，我们与客户携手为中国再生医学产业的发展而前行。
欢迎您致电 华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司
Content and Storage: Components Size Storage Shelf Life
Basal Medium (on gel ice) 450mL 2 to 8°C. Keep from light 6 months
10x Supplement (dry ice) 50mL -20°C or below. Keep from light 6 months
Additional key reagents required or suggested
Extracellular matrix proteins, such as Matrigel (BD, cat# 354277) or equivalent
Accutase (Millipore, SCR005) or Collagenase IV (Invitrogen, 17104) or equivalent
ROCK inhibitor Y-27632 (StemRD, Y-01/-05/-25) or Thiazovivin (StemRD, Thia-01/-05/-25)
Medium Preparation
1. Thaw PSGro-caseSupplement overnight at 4°C. If desired, thawed Supplement can be aliquoted and stored at -20°C or below, but further freeze-thaw should be avoided.
2. To make PSGro-casecomplete medium, add 1 part of Supplement to 9 parts of Basal Medium.
3. Antibiotics can be added to the complete medium if so desired.
4. Once made, PSGro-casecomplete medium is stable for 2 weeks when stored in dark at 2 to 8°C.
StemRD PGroF-500 PSGro-case, Human iPSC/ESC growth medium 人iPSC/ESC胚胎干细胞无血清培养基 评价◥或评论
红荣微再（上海）生¤物工程技术有限公司在中国代理销售的StemRD PGroF-500 PSGro-case, Human iPSC/ESC growth medium 人iPSC/ESC胚胎干细胞无血清培养基○是从美国进口的，由美国StemRD公司研发生产。美国StemRD公司是在经美国FDA认证的GMP实验室里面采用人类细◤胞和原核表达系统进行重组蛋白的表达生产与纯化工作，同时该GMP实验↑室拥有美国生物技术领域高度的仪器设备，确保产品质量的稳定可靠，纯度和活性优于同行，StemRD PGroF-500 PSGro-case, Human iPSC/ESC growth medium 人iPSC/ESC胚胎干细胞无血清培养基的价格∩合理，与加拿大StemCell Technologies Inc,公司的mTeSR 1 Feeder-Independent Growth of hESCs and hiPSCs人胚胎干细ㄨ胞和iPS细胞无血※清培养基相比较，可谓性价比更高。红荣微再（上海）生物工程技术有限公司在上海仓库备有少量StemRD PGroF-500 PSGro-case, Human iPSC/ESC growth medium 人iPSC/ESC胚胎干细胞无血清培养基现货，并严格按照产品的最佳储存条件进▆行储存与管理，我们的♂一贯宗旨是：更用心地为中国广大科学研究工作者提供更安全、更放心、更便捷的◆服务。
Coating plates with extracellular matrix proteins, such as Matrigel?
Refer to manufacturers’ instructions. For BD Matrigel?, the following procedure can be followed:
1. Thaw Matrigel? on ice. Dilute Matrigel 1:50 with pre‐chilled DMEM medium.
2. Immediately use the diluted Matrigel? solution to coat tissue culture plates. For a 6‐well plate, use 1 mL of diluted Matrigel? solution per well, and swirl the plate to spread the solution evenly.
3. Let the plate stand for 1 h at 37°C or overnight at 4°C.
Adaptation of growing feeder-free cells to PSGro-case
Human iPSC or ESCs that have been grown in most commonly-used hiPSC/ESC media can be easily adapted into PSGro-case. One round of 1:1 dilution of serum-containing or serum-free medium with PSGro-caseis generally sufficient. However, for certain media and cell lines, a longer and more graduate adaptation may be required.
Adaptation of growing feeder-dependent cells to PSGro-case
1. Dissociate cells with Accutase, Collagenase IV or equivalent.
2. Add the previously-used medium and gently scrape off cells as aggregates. The ideal aggregate size is similar to other media and single cells from excessive pipetting may lower plating efficiency.
3. Transfer the suspension with cell aggregates into a 15 ml tube.
4. Centrifuge at 200 x g for 5 minutes at room temperature.
5. Discard Matrigel? solution, immediately add PSGro-case(e.g., 2 mL/well for a 6-well plate).
6. After centrifugation, discard the supernatant, gently resuspend the pellet with 2 mL of the previously-used medium. Note: Adding ROCK inhibitor (Y-27632 or Thiazovivin) to the medium is shown to increase the survival and plating efficiency.
7. Transfer the cell aggregates in the previously-used medium to the wells with PSGro-case. Mix. Gently.
8. Culture the cells at 37°C, with 5% CO2 and 95% humidity.
9. If cells are seeded on Friday, change medium on Monday to 4 mL PSGro-case(no need to change over the weekend). If cells are seeded on other days during the week, change next day.
提供产品：华雅再生医学旗舰公司-红荣微再（上海）生物工程技术有限公司为中国再生医学和生物工程技术领域的组织器官再生研究、组织器官功能重建研究、干细胞技卐术研究、组织工程生物支架材料研究、器官移植医疗器械产品等再生医学产业链的相关客户提供：iPS/ES胚胎干※细胞的种子细胞、心肌细胞、人源肝脏细胞、MSC间充质干『细胞、基底膜基质胶细胞支架、干细胞生长分化调控因子、干细胞药物研究的小分子抑制剂、调节剂、小分子化合物、功能胶原蛋白◥生物材料、3D细胞培养基质〓胶、器官模型3D打印技术材料、神经干细胞培养基、STEM121抗体、BD科研P100、P800蛋白质组学采血管、BD血浆准备管PPT管、BD PAXgene全血RNA样本收集管、BD公司CPT/PRP美容采血管、StreckCell-Free RNA BCT游离RNA样本收集管、StreckCell-Free DNA Urine Preserve游离DNA尿液保存管、StreckCell-Free DNA BCT游离DNA采血管、循环肿瘤细胞CTC及无创产前基因检测采血ㄨ管、illumina高品质NGS文库制备试剂◤盒、ThruPLEX ctDNA测序试剂盒、全基因组测序、全外显子测序、靶向测序、ChIP-Seq测序、CNV和染色体异倍性分析、Rubicon Genomics单细胞全基因组测序试↘剂盒、生物医学试剂耗材仪器等种类丰富的产品，以“Focus on Tissue and Organ Regeneration专注组织器官再生与功能重建”为己任，我们与客户携手为中国再生医学产业的发展而前行。
欢迎您致电 华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司
Recovery of frozen cells in PSGro-case
1. Quickly thaw hiPSCs or hESCs in a 37°C water bath, transfer the contents to a 15 mL tube. Add 10 mL of the medium previously-used for the cells drop-wise to the tube with gentle mixing.
2. Centrifuge cells at 200 x g for 5 minutes at room temperature.
3. Discard Matrigelsolution, immediately add PSGro-case (e.g., 2 mL/well for a 6-well plate).
4. After centrifugation, discard the supernatant, gently resuspend the pellet with 2 mL of the previously-used medium. Note: Adding ROCK inhibitor (Y-27632 or Thiazovivin) to the medium is shown to increase the survival and plating efficiency.
5. Transfer the cell aggregates in the previously-used medium to the plate with PSGro-case. Mix gently.
6. Culture the cells at 37°C, with 5% CO2 and 95% humidity.
7. Change medium to 4 mL PSGro-casenext day.
Passaging cells in PSGro-caseunder feeder-free condition
1. Look under microscope to identify regions of differentiation. Mark the differentiated colonies using lens marker on the bottom of the plate (e.g., 6-well plate)
2. Remove regions of differentiation by scraping with a pipette tip or by aspiration.
3. Aspirate medium from the cell culture and rinse with PBS.
4. Add 0.5 mL per well of Accutase or Collagenase IV. Let stand at room temp for 1 – 2 min.
5. Remove Accutase, and gently rinse 2 – 3 times with 2 mL PSGro-caseto remove remaining
enzymes.
6. Add 2 mL/well PSGro-caseand scrape colonies off with a cell scraper.
7. Transfer the detached cell aggregates to a 15 mL conical tube and rinse the well with an additional
2 mL PSGro-caseto collect any remaining aggregates.
8. Centrifuge at 200 x g for 5 minutes at room temperature.
9. Aspirate the supernatant. Resuspend pellet in 4 mL PSGro-caseby pipetting gently.
Note: Adding ROCK inhibitor (Y-27632 or Thiazovivin) to the medium is shown to increase the survival and plating efficiency.
10. Plate the cell aggregates with PSGro-caseonto a new plate coated with Matrigel.
11. Culture the cells at 37°C, with 5% CO2 and 95% humidity. Change medium daily.
12. If cells are seeded on Friday, change medium on Monday to 4 mL PSGro-case(no need to change over the weekend). If cells are seeded on other days during the week, change next day.
Note: If colonies are at an optimal density, cells can be split every 5 ‐ 7 days using 1:5 to 1:10 splits.
Cryopreservation of cells in PSGro-case
1. Prepare freezing medium which contains 90% PSGro-caseand 10% DMSO.
Note: Adding ROCK inhibitor (Y-27632 or Thiazovivin) to the medium is shown to increase the survival and plating efficiency.
2. Perform steps 1 - 8 from Passaging cells in PSGro-caseunder feeder-free condition
3. Gently aspirate the supernatant taking care to keep the cell pellet intact.
4. Gently resuspend the pellet in freezing medium, taking care to leave the cell aggregates larger than would normally be done for passaging.
5. Transfer 1 mL of cell aggregates in freezing medium into each labeled cryovial.
6. Place vials into an isopropanol freezing container and place the container at ‐80°C overnight.
7. Transfer to a liquid nitrogen tank the next day for long-term storage.
红荣微再（上海）生物工程技术有限公司主营：干细胞、精准医疗、细胞治疗、器官再生四大板块的产品，以“传递科学价值，服务科学研究”为宗旨，经过近两年的努力推广，红荣微再（上海）生物工程技术有限公司在全中国代▂理的CellArtis干细胞产品已经成功销往：上海市、北京市、重庆市、天津市、广东省的广州市、深圳市、珠海市、江西省的南昌市、四川省的成都市、绵阳、浙江省的杭▅州市、宁波、温州、江苏省的南京市、苏州、无锡、泰州、湖南省的长沙、陕西省的西安市、杨凌、甘肃省的々兰州、宁夏的银川、新疆的乌鲁木齐、广西省的南宁市、山东省的济南、青岛、东北三省辽宁省的沈阳市、大连市、吉林省的长春市、黑龙ζ 江省的哈尔滨市等中国绝大部分省市和自治区。
欢迎您致电 华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司
