SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with Gene-Specific Primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or generating cDNA from total or poly (A)+ RNA sample. Like SuperScript® II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. SuperScript® III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability. The structural modifications provide:
Half life of 220 minutes at 50°C for the highest cDNA yields (Figure 1)
Reduced RNase H activity for more full-length cDNA
Full activity at 50°C for increased specificity with GSP
Ability to increase RT units without inhibiting subsequent PCR
规格
Optimal Reaction Temperature: | 50° C |
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Final Product Size(s): | 12.3 kb or less |
Reverse Transcriptase: | SuperScript® III |
Ribonuclease H Activity: | Reduced |
Form: | Frozen |
Format: | Tube(s) |
Quantity: | 10,000 units |
Template: | ssRNA |
Sensitivity: | Medium |
Product Size: | 10,000 units |
Concentration: | 200 U⁄µl |
Enzyme Function: | RNA dependent DNA Polymerase |
Shipping Condition: | Dry Ice |
Number of Reactions: | 50 Reactions |
内容及储存
SuperScript® III Reverse Transcriptase is supplied with a vial of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial of 100 mM DTT.
Store at -20°C. Guaranteed stable for 6 months when properly stored.