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为环介导等温扩增设计引物
LAMP Designer为LAMP设计高效引物，LAMP可以在等温条件下扩增DNA和RNA，避免了PCR的温度设置。这项技术依赖自动循环和DNA聚合酶介 导的链置换合成DNA，不到一小时的时间内可以将DNA从几份扩增到109份。LAMP偶联的逆转录可以用来扩增RNA序列。
LAMP使用四种特别设计的引物（两个内部和两个外部）识别靶DNA的六个显著区间。四条引∏物与DNA的杂交是决定LAMP效率的关键步骤，所以这四Ψ 条引物的设计是影响实验成功的关键。
避免交叉同源性
LAMP Designer自动注释BLAST搜索结果，并避免根据这些与数据库有显▃著交叉同源性的序列设计引物。
BLAST验证
通过BLAST搜索可↓以验证引物的特异性。
多重LAMP引物集合
为一个序列设计引物，可以进行检∞查，用于多重实验。该程序会显示出交叉同源序列和设计引物间最ζ 稳定的自由能。
结果导出
设计的引物集以及他们的属性可以导出为标准☉的CSV或Excel格式。
数据和数据库管理
多个实验数据可以通过为每一个实验创建单独的◇项目来进行管理。它可以维护包含序列信息和搜索结果的本地数据库。
Design Primers for Loop Mediated Isothermal Amplification
LAMP Designer designs efficient primers for Loop-Mediated Isothermal Amplification assays, that amplify DNA and RNA sequences at isothermal conditions, eliminating the necessity of a PCR setup. The technology relies on auto-cycling and DNA polymerase mediated strand displacement DNA synthesis, amplifying a few copies of DNA to 109 in less than an hour. Reverse transcription coupled LAMP can be applied for amplification of RNA sequences.
LAMP employs four specially designed primers (two inner and two outer) that recognize six distinct regions in the target DNA. Hybridization of the four primers to the target DNA is a very crucial step for the efficiency of LAMP. The design of these four primers is therefore critical for a successful assay.
Avoid Cross Homologies
LAMP Designer automatically interprets BLAST search results and avoids those regions to design primers that have significant cross homologies with the database.
Verification BLAST
The primers can be BLAST searched against a database to verify their specificity.
Multiplex LAMP Primer Set
The primers designed for a sequence can be checked for multiplexing. The free energies of the most stable cross homologies between the primers designed, is displayed by the program.
Export Result
The designed primer sets, along with their properties, can be exported in standard csv or excel formats.
Data & Database Management
Multiple projects can be created. Data of multiple experiments can be easily managed by creating a separate project for each experiment. It maintains a local database for sequence information and search results.
