Catalog Number:
201-02
Source:
Escherichia coli.
Molecular Weight:
7.9 kDa, a single non-glycosylated polypeptide chain containing 73 amino acids.
Quantity:
2ug/10ug/1000μg
Purity:
>97% by SDS-PAGE and HPLC analyses.
Biological Activity:
Fully biologically active when compared to standard. Determined by its ability to chemoattract hCXCR2 transfected 293 cells using a concentration range of 10.0-100.0 ng/ml.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:
Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in 20mM PB, pH 7.4, 50mM NaCl.
AA Sequence:
APLATELRCQCLQTLQGIHLKNIQSVKVKSPGPHCAQTEVIATLKNGQKACLNPASPMVKKIIEKMLKNGKSN
Endotoxin:
Less than 1EU/μg of rHuGRO-beta/CXCL2 as determined by LAL method.
Reconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage:
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Usage:
This material