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产品名称:Recombinant Human IL-1RA|重组人白介素1RA|纯度>97%|参数|价格
包装:2μg/10μg
Catalog Number: C22-101-01C
Source: Escherichia coli.
Molecular Weight: Approximately 17.1 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.
Quantity: 20μg/100μg/1000μg
AA Sequence: RPSGRKSSKM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNV NLEEKIDVVP IEPHALFLGI
HGGKMCLSCV KSGDETRLQL EAVNITDLSE NRKQDKRFAF IRSDSGPTTS FESAACPGWF
LCTAMEADQP VSLTNMPDEG VMVTKFYFQE DE
Purity: > 96 % by SDS-PAGE and HPLC analyses.
Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 40 ng/ml, corresponding to a specificactivity of > 2.5 × 10*4IU/mg in the presence of 50 pg/ml rHuIL-1α.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin: Less than 1 EU/μg of rHuIL-1RA as determined by LAL method.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to thebottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to aconcentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots andstored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage,preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. Formaximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to-70 °C. Avoid repeated freeze/thaw cycles.
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