产品名称：abscisic acid ELISA quantitation kit
产品货号：AS11 1748
产品价格：￥9841
reaction wells | 5pcs for 480 assays, 60 strips with 8 wells
tracer | 20 – 50 µl
tracer diluent | 5xstock + 0.02 % NaN3
reaction and wash solution | 10xstock+0.02 % NaN3
stopping reagent | 2x stock
substrate diluent | 10xstock +0 0.02 % NaN3
substrate | 100 mg
standards | 600 µl of each: 15.6 pmol, 7.8 pmol, 3.9 pmol, 1.95 pmol, 975 fmol, 488fmol, 244 fmol, 122 fmol, 61 fmol, 30.5 fmol, 15.2 fmol
assay development time | 4-5 hours
sensitivity |0.01 to 10 pmol/50 µl
plant extract volume | 50 µl
Assay parameters
antibody titre | 1: 5 300; unspecific binding | 2.8 %
midrange(B/Bo=50%) | 275 pmol; detection limit | 7.8 fmol, 2 pg
linear range of measurment | 15-500 fmol; intraassay variability | 4.3 %
interassay variability | 5.2 %; amount of tracer per assay | 12.4 ng
