产品名称：CometAssay Kit 96 wells
产品货号：4253-096-K
产品价格：￥3375
产品规格：96 samples
APPLICATIONS:
■Applicable for the analysis of either single strand or double strand DNA breaks
■Study cellular responses to DNA damage
■Screen for inhibitors of DNA repair
■Test chemicals for toxicity
■Screen for environmental mutagens
■Probe for specific types of DNA damage using DNA repair glycosylases
ASSAY DESIGN FOR ALKALINE COMET:
Step 1: Cells mixed with low melting agarose are immobilized on specially treated slides and lysed.
■Membranes and histones removed from the DNA of individually isolated cells.
Step 2: Samples are treated with alkali to unwind and denature the DNA.
■For detection of single and double-stranded breaks.
Step 3: Following alkaline electrophoresis slides are stained and visualized by fluorescent microscopy.
■Increase in DNA damage results in smaller fragments migrating faster in an electric field.
■Quantitative measurements of DNA damage
