产品名称：DPO-4+ Buffer + Cations
产品货号：4150-010-EB
产品价格：￥4350
产品规格：10 µg
STORAGE BUFFER:
20 mM Tris-HCl (pH 7.8), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) glycerol.
STORAGE:
Store at –20°C in a manual defrost freezer. For long-term storage, freeze at –80°C in working aliquots. Avoid repeated freeze-thawing. Enzyme may be diluted in 1X REC Buffer 15 for immediate use.
SOURCE:
Purified from E. coli containing a recombinant plasmid harboring the S. solfataricus Dpo4 gene.
UNIT DEFINITION:
One unit of enzyme incorporates 1 nmole of dTMP onto the 3 end of d(T) 24 using poly(dA)300 as the template in 30 minutes at 60°C.
READ-THROUGH SPECIFICITY:
Dpo4 catalyzes translesion synthesis across the following forms of DNA damage: AP sites, cis-syn thymine-thymine dimer, 6-4 photoproducts, cisplatinum- and acetyl-aminofluorene guanine adducts.
