产品名称：Human ß Polymerase Enzyme & Buffer
产品货号：4020-01K-EB
产品价格：￥13425
产品规格：1000 units
SOURCE:
Purified from E. coli containing a recombinant plasmid harboring the human DNA polymerase β gene.
UNIT DEFINITION:
One Unit is the amount of enzyme required to catalyze the incorporation of 1 nmole of dNTP into an acid-insoluble form in 1 hour at 37°C.
SPECIFICITY:
The enzyme can fill small gaps (up to 6 nucleotides) and nicks in DNA, catalyze DNA synthesis after base excision repair, and release 5-terminal deoxyribose phosphate residues from incised AP sites.
ASSAY CONDITIONS:
1X REC Buffer 8 (50 mM Tris-Cl (pH 8.8), 10 mM MgCl2, 10 mM KCl, 1.0 mM DTT, 1%glycerol) 50 μM dCTP, 50 μM dGTP, 50 μM dATP, 50 μM α-32P-dTTP, and 100 μg/ml ofActivated DNA (Catalog # 4667-50-06 see page 128) in a reaction volume of 100 μl are incubated for 5 min at 37°C.
STORAGE BUFFER:
20 mM Tris-Cl (pH 7.8), 1.0 mM DTT, 1 mM EDTA, 100 mM NaCl, and 50% (v/v) glycerol.
STORAGE:
Store at -20°C in a manual defrost freezer.
