产品名称：b-Gal ELISA产品货号：产品价格：￥6937产品规格：1 kit (192 tests)Application:The β-Gal ELISA is used to quantitatively measure β-Gal expression in eukaryotic cells that are transfected with a plasmid bearing a β-Gal-encoding reporter gene.Benefits:?Standardized: Allows direct comparison of data from different sets of experiments, even when kits from different production lots are used?More sensitive: More sensitive than colorimetric β-Gal assays?More accurate: Measures the actual amount of β-Gal protein synthesized, not just β-Gal enzyme activity?More specific: Detects bacterial, not endogenous β-Gal?Fast, approximately 4 hours elapse from start to finish?Easy to perform: Follows a standard ELISA protocol
产品名称：b-Gal ELISA
产品货号：
产品价格：￥6937
产品规格：1 kit (192 tests)
Application:
The β-Gal ELISA is used to quantitatively measure β-Gal expression in eukaryotic cells that are transfected with a plasmid bearing a β-Gal-encoding reporter gene.
Benefits:
•Standardized: Allows direct comparison of data from different sets of experiments, even when kits from different production lots are used
•More sensitive: More sensitive than colorimetric β-Gal assays
•More accurate: Measures the actual amount of β-Gal protein synthesized, not just β-Gal enzyme activity
•More specific: Detects bacterial, not endogenous β-Gal
•Fast, approximately 4 hours elapse from start to finish
•Easy to perform: Follows a standard ELISA protocol
Principle:
The β-Gal ELISA is based on the sandwich ELISA principle. Following lysis of the transfected cells, the cell extracts (containing the β-Gal enzyme) are added to the wells of the microplate, which are precoated with a polyclonal antibody to β-Gal (anti-β-Gal). All β-Gal contained in the cell extracts binds to the anti-β-Gal antibody that is bound to the microplate surface. Next, a digoxigenin-labeled antibody to β-Gal (anti-β-Gal DIG) is added, then bound to the β-Gal. In the next step, an antibody to digoxigenin conjugated to peroxidase (anti-DIG-POD) is added, then bound to digoxigenin. In the final step, the peroxidase substrate (ABTS) is added. The peroxidase enzymes catalyze the cleavage of the substrate to produce a colored reaction product. Absorbance of the samples is determined using an ELISA reader, and is directly correlated to the level of β-Gal present in the cell extract. The sensitivity of the assay can be enhanced using ABTS with a substrate enhancer.
Product Description:
Assay time: approx. 4 hours
Sample material: Cell extracts
Sensitivity: ≥30 pg/ml (6 pg/well)
Specificity: The test is specific for β-Gal (β-D-galactoside galactohydrolase, EC 3.2.1.23) from E. coli. It does not crossreact with eukaryotic, lysosomal β-galactosidase.
Standard: The β-Gal enzyme from E. coli, included in the kit for the purpose of compiling a standard calibration curve, is provided with lot-specific content data as determined by immunoassay.
