产品: invitrogen
货号: K414001
规格: 1 KIT
名称: DES BIOEASE KIT WITH PCOBLAST
The DES? BioEase? Expression Kit contains 6 μg of the pMT/BioEase?-DEST vector, 10 μg of the pMT/BioEase?-GW/lacZ expression control, and 20 μg of the pCoBlast selection vector. It also contains the Calcium Phosphate Transfection Kit, frozen S2 cells, GIBCO? Schneiders Drosophila Medium, and 50 mg of Blasticidin. Store S2 cells in liquid nitrogen. Store medium at +4°C. Store all other components at -20°C. All components are guaranteed stable for 6 months when properly stored.
The DES? BioEase? Expression Kit with pCoBlast provides an easy, efficient method for expressing, purifying, and detecting biotinylated recombinant proteins. The pMT/BioEase?-DEST vector incorporates a 72-amino acid sequence from K. pneumoniae that directs in vivo biotinylation of a specific lysine residue. Proteins produced in pMT/BioEase?-DEST are expressed as fusion proteins to this sequence. Biotinylated fusion proteins can be purified on streptavidin agarose or detected on