【产品名称】Actin, cAb (S12-I)
【供应商】Alexis
【货号】ALX-810-001-R100
【规格】100 µl
【产品说明】
Clone: S12-I
Host: Rabbit
Isotype: IgG
Immunogen: Synthetic peptide corresponding to a C-terminal sequence of human β-actin.
Application: ELISA (1:10’000-1:20’000)
Immunoprecipitation
Western Blot (1:2’000)
Wash buffer: 1x TRIS buffered saline (TBS) containing 0.1% Triton X-100
Blocking buffer: 1x TBS containing 0.1% Triton X-100 and 5% nonfat dry milk.
Incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature.
Optimal conditions must be determined individually for each application.
Specificity: Recognizes human, mouse and rat α-actin and β-actin.
Purity Detail: Major clone of IgGs obtained from immunoaffinity purified immunoglobulins corresponding to immunogenic peptide.
Formulation: Liquid. In 20mM TRIS/HCl, pH 8.0, containing 10mg/ml BSA and 0.05% sodium azide.
Concentration: 1.9mg/ml
Use/Stability: Stable for at least 18 months after receipt when stored as recommended.
Handling: After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Long Term Storage: -20°C