产品简介：
细胞内PH值在许多细胞生命活动中发挥着重要作用，例如细胞生长，钙离子的调控，胞吞作用，趋化性，细胞粘合和其它细胞活动。BCECF被广泛地用于细胞内pH的测定。Tsien博士和其他人通过加入2个额外的羧化物基团改进了这种羧基荧光素。加入的两个羧基使得它能更好地被固定在细胞内。BCECF具有很好的水溶性，是因为它在中性pH时带有4到5个负电荷。但是BCECF却很难穿过细胞╲膜进入到细胞内。它的pKa值为6.97，高于其它的羧基荧光素。BCECF在〓激发光谱439 nm处有等吸收点，因此它能¤和Fura 2一样可用比率法测定。505 nm和439 nm通常是非常有用的用于比◇率测量的波长，一般会将490 nm和450 nm的滤光片加在激发光源的前面。而530 nm的滤光片则是用来查看它的荧光信号的。这里要注意的是，激发光谱与吸Ψ 收光谱有一些细微的区别。BCECF-AM是乙酰甲酯化的BCECF。它能够轻易地进入到细胞内▅。就和其他的乙酰甲酯类一样，BCECF-AM只需要通过孵育就能进入细胞∞。
英文注释：
Intracellular pH plays an important modulating role in many cellular events, including cell growth, calcium regulation, enzymatic activity, receptor-mediated signal transduction, ion transport, endocytosis, chemotaxis, cell adhesion and other cellular processes. pH-sensitive fluorescent dyes have been widely applied to monitor changes in intracellular pH in recent years. Imaging techniques that use fluorescent pH indicators also allow researchers to investigate these processes with much greater spatial resolution and sampling density that can be achieved using other technologies such as microelectrode. Among them, 2,7-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) is the most popular pH probe since it can be used to monitor cellular pH ratiometrically. BCECF AM is the cell-permeable version of BCECF.
相关产品：
|
产品名称 |
货号 |
规格 |
|
21189 |
1 mg |
|
|
RatioWorks? BCFL, AM *Superior Replacement to BCECF* |
21190 |
1 mg |
|
RatioWorks? BCFL, SE *Superior Replacement to BCECF* |
21191 |
1 mg |
|
pH荧光探针BCECF酸[ 85138-49-4] |
21201 |
1 mg |
|
21202 |
1 mg |
|
|
pH荧光探针BCECF, AM *超级纯* |
21203 |
20x50 ug |
|
21180 |
500 Tests |
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