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                P815(P815(肥大细胞瘤细胞)

                P815(P815(肥大细胞瘤细胞)
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                • P815(P815(肥大细胞瘤细胞)
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                ATCC
                TIB-64 (株)
                上海锐聪科技发展有限公司
                上海
                详细说明

                P815(P815(肥大细胞瘤细胞)

                P815(肥大细胞瘤细胞)
                 
                Cell Biology
                ATCC® Number: TIB-64™ Price: $203.00
                Designations:
                P815
                Depositors:
                P Ralph
                1
                Shipped:
                frozen
                Medium & Serum:
                Growth Properties:
                suspension (some adherent cells)
                Organism:
                Mus musculus (mouse)
                   
                 
                Source:
                Cell type: mast cell
                Disease: mastocytoma
                Cellular Products:
                lysozyme [1080]
                Permits/Forms:
                In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
                Applications:
                transfection host (technology from amaxa)
                Strain:
                DBA/2
                Comments:
                P815 cells phagocytose latex beads but not zymosan or BCG.
                They do not function in antibody dependent cell mediated cytotoxicity.
                Growth of the cells is not inhibited by dextran sulfate, LPS or PPD. [1136] [2104]
                Tested and found negative for ectromelia virus (mousepox).
                Propagation:
                ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
                Temperature: 37.0C
                Subculturing:
                Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Adherent cells can be recovered by scraping.
                Medium renewal: Every 2 to 3 days
                Preservation:
                culture medium 95%; DMSO, 5%
                Related Products:
                Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2002
                recommended serum: ATCC 30-2020
                References:
                1080: Ralph P , et al. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976. PubMed:
                1135: Ralph P , Nakoinz I . Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031
                1136: Ralph P , Nakoinz I . Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PubMed: 318922
                1137: Ralph P , Nakoinz I . Lipopolysaccharides inhibit lymphosarcoma cells of bone marrow origin. Nature 249: 49-51, 1974. PubMed:
                2104: Ralph P , et al. Lymphosarcoma cell growth is selectively inhibited by B lymphocyte mitogens: LPS, dextran sulfate and PPD. Biochem. Biophys. Res. Commun. 61: 1268-1275, 1974. PubMed:
                22262: Lundak RL , Raidt DJ . Cellular immune response against tumor cells. I. In vitro immunization of allogeneic and syngeneic mouse spleen cell suspensions against DBA mastocytoma cells. Cell. Immunol. 9: 60-66, 1973. PubMed:
                22825: Plaut M , et al. Studies on the mechanism of lymphocyte-mediated cytolysis. IV. Specificity of the histamine receptor on effector T cells. J. Immunol. 111: 389-394, 1973. PubMed:
                29033: Schmidt W , et al. Cell-free tumor antigen peptide-based cancer vaccines. Proc. Natl. Acad. Sci. USA 94: 3262-3267, 1997. PubMed:
                32502: Gonzalez Armas JC , et al. DNA immunization confers protection against murine cytomegalovirus infection. J. Virol. 70: 7921-7928, 1996. PubMed:

                 

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