名称:葡萄糖苷酸酶检测底物
英语名:(Aldouronic Acid Mixture Substrate for a-glucuronidase)
规格:120mg
产地:爱尔兰
外观 液体
比重 不适用
在溶于水称为易溶解度
pH值 3.5
气味 无
状 液体
稳定 在冷冻稳定超过5年
成分:
名称 比例
Aldouronic酸（从桦木木聚糖） 10％
水 90％
Aldouronic Acid Mixture
(Contains a mixture of aldotetraouronic, aldotriouronic and aldobiouronic acids; 40:40:20). Substrate for a-glucuronidase.
Catalogue Number: O-AMX
Content: 120 mg
Appearance Liquid
Specific Gravity Not applicable
Solubility in Water Readily soluble
pH Value 3.5
Odour None
Form Liquid
Stability Stable in a freezer for more than 5 years.
Ingredients Name CAS Proportion
Aldouronic Acids (from Birchwood Xylan) 10%
Water 90%
PREPARATION:
Aldo-uronic acids are prepared by the controlled acid hydrolysis of glucurono-xylan, followed
by chromatographic separation of the oligomers.
PURITY:
The aldo-uronic acids are devoid of xylosaccharides, but each individual oligomer contains some
of the higher and lower homologue.These charged oligosaccharides are difficult to completely
separate chromatographically.
The thin layer chromatographic pattern of the oligomers is shown below. Chromatography is
in ethyl acetate: acetic acid: water (2:1:1, once).The chromatographic pattern of a mixture of
borohydride reduced aldo-uronic acids is also shown.This mixture is a potential substrate for
a-glucuronidase.The 13C-n.m.r patterns for aldotriouronic acid is as described by Cavagna,
F., Deger, H. and Puls, J. (1984) Carbohydrate Research, 129, 1-8.
PRODUCT AS SUPPLIED:
Aldo-uronic acids are quite unstable at neutral pH. Consequently, they are supplied in solution in
10 mM ammonium formate (pH 3.0) or in 100mM acetic acid.These should be stored frozen on
receipt.The borohydride reduced aldo-uronic acids are more stable.These are supplied in a
freeze-dried form (as a hydroscopic syrup). This should also be stored at - 20°C on receipt.
THIN LAYER CHROMATOGRAPHY OF ALDO-URONIC ACIDS:
1. Aldo-biouronic acid standard (O-ABIO)
2. Aldo-triouronic acid (~85% pure)
3. Aldo-tetraouronic acid (~ 80% pure)
4. Reduced aldo-uronic acids.
Lot 80601.(tri:tetra:penta = 40:40:20) (OAMXR).
A potential substrate for the assay of
a-glucuronidase.
5. Aldo-uronic acid mixture. Lot 10801.
Contaminants in the oligosaccharides are
higher and lower dp homologues.
