Less toxic alternative to PMSF, pH stable.
AEBSF irreversibly inactivates thrombin and other serine proteases (e. g. chymotrypsin, kallikrein, plasmin, proteinase K, trypsin) by sulfonylation of a functional group in the active center of the enzyme. AEBSF (LD50 oral, mouse 2.8g/kg) is a substantially less toxic substitute for PMSF and DFP